1. 暨南大学生态学系, 广东省高校水体富营养化与赤潮防治重点实验室, 广州 510632; 2. 梅州市环境监测中心, 广东省梅州市 514000; 3. Environmental and Analytical Laboratories, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
Toxic effect of nonylphenol on Phaeodactylum triconutum and its mechanism
LI Gen, GUAN Chao, AN Min, DUAN Shun-shan*
1. Department of Ecology, Jinan University ; Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institutes, Guangzhou 510632,China; 2. The Meizhou Environmental Monitoring Center, Meizhou Guangsdong 514000,China; 3．Environmental and Analytical Laboratories, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
In order to explore the toxic effects of nonylphenol (NP) on marine microalgae, effects of NP on the growth of Phaeodactylum triconutum were studied. The exposure concentrations of NP were 0, 0.2, 0.4, 0.6, 0.8, 1.0 and 2.0 mg·L-1. Growth, photosynthetic pigment contents, soluble protein contents, MDA contents and Fv/Fm, as well as 24h, 48h, 72h and 96h EC50 were measured during the experimental period. The results showed that, the 96 h EC50 of NP for P. triconutum was 0.84 mg·L-1. When the exposure concentration of NP was above 0.4 mg·L-1, the growth of P. triconutum was inhibited. And when it was more than 1.0 mg·L-1, there was mass mortality of the algal cells. No growth of P. triconutum was observed as it came to 2.0 mg·L-1. The decreases of growth, photosynthetic pigment contents, soluble protein contents, MDA contents, Fv/Fm were more notable with the increasing exposure concentration of NP. After 24 h exposure treatment, MDA contents increased with the increase of concentration of NP, indicating that high NP concentration enhanced algal cell membrane lipid peroxidation, and thus led to the structural damage and functional loss of cell membrane structure. NP had obvious toxic effects on the growth of P. tricornutum, and antioxidant enzyme systems and photosynthetic system were damaged.
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