Effect of combined exposure to dimethylphthalate and diethylphthalate on growth of Dunaliella salina
HE Dong-mei, QIAN Xiao-jia, DUAN Shun-shan
Department of Ecology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou, 510632, China
Combined toxic effect may result from the concurrent exposure of multiple toxicants.In this study,the combined toxic effect of dimethylphthalate(DMP) and diethylphthalate(DEP) on growth of Dunaliella salina was evaluated by taking the 96h EC50as one toxic unit(TU).The single toxic effect as well as combined toxic effect on the biomass,soluble protein content and photosynthetic pigment content of D.Salina was detected based on the different toxicity unit proportion of 1:1,1:4,and 4:1.The results showed that both DMP and DEP inhabited the growth of D.Salina.Moreover,the toxic effect of simultaneous exposure was more significant than single exposure of the two chemicals.The values of 96h EC50 of DMP and DEP on D.Salina were 317.49mg·L-1and 69.54mg·L-1,respectively.Synergetic effect was observed for the toxicity of DMP and DEP in three toxicity unit proportions,which was more obvious in the proportion of 1:1.
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