邻苯二甲酸二乙酯和壬基酚联合暴露对杜氏盐藻生长的影响

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摘要

为了探讨环境激素类物质邻苯二甲酸二乙酯(DEP)和壬基酚(NP)对海洋微藻的联合毒性效应,选取杜氏盐藻(Dunaliella salina)为受试生物,以环境激素对杜氏盐藻单一暴露的96h EC₅₀的毒性效应作为一个毒性单位(IU),采用毒性单位法比较研究了DEP和NP单一暴露以及两者以三种不同混合比例(毒性单位比:1:1、1:4和4:1)暴露对杜氏盐藻的细胞生长、叶绿体色素含量、可溶性蛋白含量、SOD活性以及最大光能转化效率(Fv/Fm)的影响.实验结果表明:DEP和NP单一暴露对杜氏盐藻的96h EC₅₀分别为69.54 mg/L和1.47 mg/L,两种环境激素对杜氏盐藻均有抑制作用,且NP较DEP对杜氏盐藻的毒性更强.DEP和NP联合暴露较单一暴露对杜氏盐藻的细胞生长、叶绿体色素和可溶性蛋白的合成有较强的抑制作用,两种环境激素在毒性单位比为1:1、1:4、4:1三个比例水平上的联合毒性效应均表现为协同效应,其中比例为1:1的协同效应最强.

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	王晶晶
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	段舜山

关键词 ：邻苯二甲酸二乙酯, 壬基酚, 杜氏盐藻, 联合毒性效应

Abstract：

Combined toxic effect of diethylphthalate(DEP)and nonylphenol(NP)on the growth of Dunaliella salina was evaluated by taking the 96h EC₅₀ as one toxic unit(TU).Cell density,protein content,chloroplast pigment content,SOD activity,and maximal photochemical efficiency of PSⅡ(Fv/Fm)of D.salina were examined after the single and three different combined exposure for 96h (toxic unit proportion 1:1,1:4,4:1).The results showed that both DEP and NP inhabited the growth of D.salina.Moreover,the toxic effect of simultaneous exposure was more significant than single exposure of the two chemicals.The values of 96h EC₅₀ of DEP and NP on D.salina were 69.54 mg/L and 1.47 mg/L,respectively.The single toxic effect as well as combined toxic effect on the biomass, soluble protein content,chloroplast pigment,and SOD activity content of D.salina was detected based on the different toxicity unit proportions.Synergetic effect was observed for the toxicity of DEP and NP in the three toxicity unit proportions,which was more obvious in the proportion of 1:1.

Key words： diethylphthalate nonylphenol Dunaliella salina combined toxic effect

收稿日期: 2012-08-25

Q17

基金资助:NSFC-广东联合基金重点项目(U1133003);国家自然科学基金项目(41176104)

引用本文:

王晶晶1, 钱晓佳1, 安民2, 段舜山1*. 邻苯二甲酸二乙酯和壬基酚联合暴露对杜氏盐藻生长的影响[J]. , 2012, 31(4): 370-376.
WANG Jing-jing1, QIAN Xiao-jia1, AN Min2, DUAN Shun-shan1. Effect of combined exposure of diethylphthalate and nonylphenolon on growth of Dunaliella salina. , 2012, 31(4): 370-376.

链接本文:

http://www.ecolsci.com/CN/Y2012/V31/I4/370

[1] 邱海源,王宪.邻苯二甲酸酯对海洋藻类的致毒效应[J].厦门大学学报(自然科学版),2005,44(2):294-296.

[2] 王连生.环境污染化学[M].北京:化学工业出版社,1995.316-318.

[3] 胡晓宇,张克荣,孙俊红,吴德生.中国环境中邻苯二甲酸酯类化合物污染的研究[J].中国卫生检验杂志,2003,13(2):9-14.

[4] Lye C M,Frid C L J,Gill M E,Cooper D W,Jones D M.Estrogenic Alkylphenols in Fish Tissues,Sediments,and Waters from the UK Tyne and Tees Estuaries[J].Environmental Science and Technology,1999,33:1009-1014.

[5] Soares A,Guieysse B,Jefferson B,Cartmell E,Lester J N.Nonylphenol in the environment:a critical review on occurrence,fate,toxicity and treatment in wastewaters[J].Environment International,2008,34(7):1033-1049.

[6] Frassinetti S,Barberio C,Caltavuturo L,Fava F,Gioia D D.Genotoxicity of 4-nonylphenol and nonylphenol ethoxylate mixtures by the use of Saccharomyces cerevisiae D7 mutation assay and use of this text to evaluate the efficiency of biodegradation treatments[J].Ecotoxicology and Environmental Safety,2011,74(3):253-258.

[7] 吴海珍,梁世中,韦朝海.壬基酚环境行为及生物降解研究进[J].化工环保,2006,26(1):31-34.

[8] 杜青平,黄彩娜,贾晓珊,袁保红.1,2,4-三氯苯对3种海洋微藻的毒性效应[J].生态环境,2007,16(2):352-357.

[9] 黄健,韩德婷,唐学玺,李永祺.多环芳烃对2种海洋微藻的联合毒性效应研究[J].青岛海洋大学学报,2003,33(1):71-74.

[10] 吴燕燕,李来好,刁石强,杨贤庆,陈胜军,岑剑伟,周婉君.环境激素对水产品安全性的影响及预防[J].海洋科学,2008,32(1):94-96.

[11] Pamela A Pape-Lindstrom,Michael J Lydy.Synergistic toxicity of atrazine and organophosphate insecticides contravenes the response addition mixture model[J].Environmental Toxicology and Chemistry,1997,16(11):2415-2420.

[12] 丛海兵,黄廷林,周真明.藻类叶绿素测试新方法[J].给水排水,2007,33(6):28-32.

[13] 张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003.67-69.

[14] Yu Q,Rengel Z.Micronutrient deficiency influences plant growth and activities of superoxide dismutases in narrow-leafed lupins[J].Annals of Botany,1999,83:175-182.

[15] 沈宏,周培疆.环境有机污染物对藻类生长作用的研究进展[J].水生生物学报,2002,26(5):529-535.

[16] 国家环境保护总局.中华人民共和国环境保护行业标准:新化学物质危害评估导则[S].北京:中国环境科学出版社,2004.12.

[17] 张征,吴振斌.酞酸酯对水生生物的生物学效应[J].淡水渔业,2006,36(1):57-59.

[18] 阎海,叶常明,雷志芳,颜文红.蛋白核小球藻与DEP的相互作用[J].环境科学,1995,16(1):23-26.

[19] 孙禾琳,蔡恒江,姚子伟.微板法测试12种POPs对新月菱形藻的毒性[J].海洋科学进展,2011,29(4),529-536.

[20] 管超,孙志伟,安民,段舜山.壬基酚对球形棕囊藻的生态毒性效应[J].生态环境学报,2011,20(4):640-645.

[21] Servos M R.Review of the aquatic toxieity,estrogenic responses and bioaccumulation of alkylphenols and polyethox ylates[J].Water Quality Reseach Journal of Canada,1999,34(1):123-177.

[22] Marking L L.Toxicity of chemical mixtures[M].Fundamentals of aquatic toxicology.Hemisphere,New York,1985:164-176.

[23] 吴志辉,聂湘平,杨宇峰.邻苯二甲酸二甲酯(DMP)对海洋微藻的生态毒理研究[J].生态科学,2006,25(2),168-170.

[24] Qian H,Li J,Chen W,Wang J,Zhou Z,Liu W,Fu W.The effect of exogenous nitric oxide on alleviating herbicide damage in Chlorella vulgaris[J].Aquatic Toxicology,2009,92(4):250-257.

[25] Geoffroy L,Dewez D,Vernet G,Popovic R.Different physiological parameters used in evaluation of oxyfluorfen effect on S.obliquus:validity of parameters as biomarkers[J].Archives of Environmental Contamination and Toxicology,2003,45(4):439-454.

[26] 杨良,葛飞,喻方琴,许银,朱润良,朱门君,王静.十六烷基三甲基氯化铵与荧蒽对小球藻的联合毒性及其作用机制[J].中国环境科学,2011,31(1):150-155.

[27] Rontain J F,Aubert C.Effect of oxy-free radicals upon the phytyl chain during Chlorophyll a photodegration[J].Journal of Photochemistry and Photobiology A:Chemistry,1994,79(3):167-172.

[28] Rontain J F.Visible light-dependent degration of lipidic phytoplanktonic component during senescence:a review[J].Phytochemistry,2001,58(2):187-202.