大亚湾海域可培养细菌鉴定及系统发育分析

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (348 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

对大亚湾海域表层水体的可培养细菌进行分离,测定了分离培养细菌的16S rRNA 基因V3 区序列,并采用该研究中的35 个序列与从GenBank 下载的46 个序列构建了系统树,对细菌进行了分子鉴定。在9 个站点的表层水体中共分离得到70株菌株,分属35 个种。其中α-变形菌纲(Alphaproteobacteria)和放线菌纲(Actinobacteria)为分离菌株最多的两个菌纲,分离率分别为40%和35.7%;而β-变形菌纲(Betaproteobacteria)仅分离到1 株(1.4%)。此外,还分离得到盖球菌属(Kytococcus)、间胞囊菌属(Intrasporangium)等非常见菌株。大亚湾海域细菌的Shannon-Wiener's 多样性指数为3.21。大亚湾海域表层水体可培养细菌丰富的种类多样性以及较高的α-变形菌分离率,说明了该海域具有良好的水质,同时也是潜在的微生物菌种资源库。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	江晓亮
	王朝晖
	王剑
	熊毅俊
	林朗聪

关键词 ：大亚湾, 可培养细菌, 16S rRNA 基因, 系统发育分析

Abstract：

Culturable bacteria were isolated from surface seawater in Daya Bay, and identified by means of 16S rRNA gene V3 region sequences. A phylogenetic tree was constructed using 35 sequences in this study and 46 sequences from GenBank. Seventy bacterial isolates were isolated and cultured from surface water of 9 stations in Daya Bay, which were classified into 35 categories. Within them, 40% belonged to Alphaproteobacteria, 35.7% to Actinobacteria, and 1.4% to Betaproteobacteria. Meanwhile, two genera, Kytococcus and Intrasporangium, which were rarely reported, were also isolated. The value of Shannon-Wiener`s index was 3.21, indicating a diversity of bacteria in Daya Bay. The high diversity of the culturable bacteria and abundance of Alphaproteobacteria suggested the good water quality and the potential microbial resources in Daya Bay.

Key words： Daya Bay culturable bacteria 16S rRNA gene phylogenetic analysis

收稿日期: 2013-06-25

983

引用本文:

江晓亮, 王朝晖*, 王剑, 熊毅俊, 林朗聪. 大亚湾海域可培养细菌鉴定及系统发育分析[J]. , 2013, 32(3): 290-297.
JIANG Xiao-liang, WANG Zhao-hui*, WANG Jian, XIONG Yi-jun, LIN Lang-cong. Identification and phylogenetic analysis of culturable bacteria in Daya Bay. , 2013, 32(3): 290-297.

链接本文:

http://www.ecolsci.com/CN/Y2013/V32/I3/290

[1] 张志南, 田胜艳. 异养细菌在海洋生态系统中的作用[J]. 青岛海洋大学学报, 2003, 33(3): 375-383.
[2] Rabus R, Nordhaus R, Ludwig W, Widdel F. Complete oxidation of toluene under strictly anoxic conditions by a new sulfate-reducing bacterium[J]. Applied and environmental microbiology, 1993, 59(5): 1444-1451.
[3] Selvin J, Priya S S, Kiran G S, Thangavelu T, Bai N S. Sponge-associated marine bacteria as indicators of heavy metal pollution[J]. Microbiological Research, 2009, 164(3): 352-363.
[4] Skanavis C, Ieee. Suitable indicator for the presence of sewage sludge in marine sediments[J]. Oceans'98-Conference Proceedings, 1998, 1-3: 1844-1853.
[5] Wu J Y, Jiang X T, Jiang Y X, Lu S Y, Zou F, Zhou H W. Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method[J]. BMC Microbiology, 2010, 10(1): 255.
[6] Celussi M, Cataletto B. Annual dynamics of bacterioplankton assemblages in the Gulf of Trieste (Northern Adriatic Sea)[J]. Gene, 2007, 406(1-2): 113-123.
[7] 陈文静, 周劲风, 李耀初. 大亚湾海域水质变化趋势及成因分析[J]. 环境科学与技术, 2010, 33(12F): 28-32.
[8] 陈明霞, 李和阳, 李刚, 郑天凌, 焦昆, 韩永胜. 深圳海域弧菌种类组成、数量分布及其与环境因子的关系研究[J]. 海洋学报, 2010, 32(5): 117-126.
[9] 李和阳, 陈明, 李刚, 郑天凌, 郑森林, 陈彬. 深圳海域细菌总数及可培养细菌总数的分布及其在环境评价中的应用研究[J]. 海洋环境科学, 2011, 30(4): 487-491.
[10] Jiang X, Ren C h, Hu C q, Luo P, Chen C, Feng J b. On dynamic change of Vibrio species in the Daya Bay using molecular identification method[J]. Journal of Tropical Oceanography, 2010, 29(4): 154-159.
[11] Hagström R, Pinhassi J, Zweifel U L. Biogeographical diversity among marine[J]. Aquat Microb Ecol, 2000, 21: 231-244.
[12] M. K. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences[J]. J Mol Evol, 1980, 16: 111-120.
[13] Hill T C J, Walsh K A, Harris J A, Moffett B F. Using ecological diversity measures with bacterial communities[J]. Fems Microbiology Ecology, 2003, 43(1): 1-11.
[14] Agogue H, Casamayor E O, Bourrain M, Obernosterer I, Joux F, Herndl G J, Lebaron P. A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems[J]. FEMS Microbiol Ecol, 2005, 54(2): 269-280.
[15] Eilers H, Pernthaler J, Peplies J, Glockner F O, Gerdts G, Amann R. Isolation of novel pelagic bacteria from the German bight and their seasonal contributions to surface picoplankton[J]. Applied and Environmental Microbiology, 2001, 67(11): 5134-5142.
[16] Schmidt T M, DeLong E F, Pace N R. Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing[J]. Journal of bacteriology, 1991, 173(14): 4371-4378.
[17] Bano N, Hollibaugh J T. Phylogenetic composition of bacterioplankton assemblages from the Arctic Ocean[J]. Applied and Environmental Microbiology, 2002, 68(2): 505-518.
[18] Eilers H, Pernthaler J, Glockner F O, Amann R. Culturability and In Situ Abundance of Pelagic Bacteria from the North Sea[J]. Applied and Environmental Microbiology, 2000, 66(7): 3044-3051.
[19] Tamaki H, Sekiguchi Y, Hanada S, Nakamura K, Nomura N, Matsumura M, Kamagata Y. Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques[J]. Appl Environ Microbiol, 2005, 71(4): 2162-2169.
[20] 戴欣, 王保军, 黄燕, 张平, 刘双江. 普通和稀释培养基研究太湖沉积物可培养细菌的多样性[J]. 微生物学报, 2005, 45(2): 161-165.
[21] Spring. S, Schulze. R, Overmann. Jr, Schleifer. K H. Identification and characterization of ecologically significant prokaryotes in the sediment of freshwater lakes, molecular and cultivation studies[J]. FEMS Microbiology Reviews, 2000, 24: 573-590.
[22] Ling J, Dong J D, Wang Y S, Zhang Y Y, Deng C, Lin L, Wu M L, Sun F L. Spatial variation of bacterial community structure of the Northern South China Sea in relation to water chemistry[J]. Ecotoxicology, 2012, 21(6): 1669-1679.
[23] Wobus A, Bleul C, Maassen S, Scheerer C, Schuppler M, Jacobs E, RÃ¶ske I. Microbial diversity and functional characterization of sediments from reservoirs of different trophic state[J]. FEMS Microbiology Ecology, 2003, 46(3): 331-347.
[24] Lee J H, Shin H H, Lee D S, Kwon K K, Kim S J, Lee H K. Bacterial diversity of culturable isolates from seawater and a marine coral, Plexauridae sp., near Mun-Sum, Cheju-Island[J]. The Journal of Microbiology, 1999, 37(4): 193-199.
[25] 吴根福, 虞左明, 吴洁, 周雪平. 杭州西湖水域可培养异养细菌的群落多样性[J]. 生物多样性, 2003, 11(6): 467-474.
[26] Graciela N P, Adrián J A. Diversity of culturable marine bacteria on the coastline of the central area of San Jorge Gulf, Argentina[J]. Revista de Biología Marinay Oceanografía, 2012, 47(2): 367-371.
[27] Frette L, Johnsen K, Jorgensen N O G, Nybroe O, Kroer N. Functional characteristics of culturable bacterioplankton from marine and estuarine environments[J]. International Microbiology, 2004, 7(3): 219-227.
[28] 李潞滨, 刘敏, 杨淑贞, 刘亮, 缪崑, 杨凯, 韩继刚. 毛竹根际可培养微生物种群多样性分析[J]. 微生物学报, 2008, 48(6): 772-779.
[29] 孙风芹, 汪保江, 李光玉. 南海南沙海域沉积物中可培养微生物及其多样性分[J]. 微生物学报, 2008, 48(12): 1578-1587.
[30] 李卓佳, 郭志勋, 冯娟, 张汉华, 杨莺莺. 应用芽孢杆菌调控虾池微生态的初步研究[J]. 海洋科学进展, 2006, 30(11): 28-31.
[31] Graciela N P, Adrián J A. Diversity of culturable marine bacteria on the coastline of the central area of San Jorge Gulf, Argentina[J]. Revista de Biología Marinay Oceanografía, 2012, 47: 367-371.
[32] 贾燕, 尹华, 彭辉, 叶锦韶, 秦华明, 张娜. 石油降解菌株的筛选、初步鉴定及其特性[J]. 暨南大学学报(自然科学版), 2007, 28(3): 296-301.
[33] 袁国明, 何桂芳. 大亚湾水环境质量变化与环境容量评估[J]. 台湾海峡, 2012, 31(4): 472-478.
[34] 梁珊珊, 张兵, 李俊生, 张浩, 申茜. 环境一号卫星热红外数据监测核电站温排水分布—以大亚湾为例[J]. 遥感应用, 2012: 41-46.
[35] Tam L, Kevan P G, Trevors J T. Viable bacterial biomass and functional diversity in fresh and marine waters in the Canadian Arctic[J]. Polar Biology, 2003, 26(5): 287-294.