The effect of pretreatment.and preservation procedures on carbon and nitrogen stable isotope ratios of zooplankton Phyllodiaptomus tunguidus and Diaphanosoma orghidani transamurensis was studied.The results showed that gut contents in P.tunguidus led to a significant deviation in the values of replicates of both δI3C and δI5N.Lipid extraction resulted in a significant enrichment in δI3C and δI3N in P.tunguidus.Methanol and ethanol preserved animals showed higher δI3C values,while the frozen and formalin-preserved animals resulted in lower δI3C values.δI5N of two species was affected slightly,but not significantly,by four different preservations in this study.The results suggest that the variation from different treatments must be considered when making cross-study comparisons.Standard protocols for sample preparation and preservation are needed for the study of foodweb structure via stable isotope analysis.
韩龙1*, 刘正文1,2. 预处理及保存方法对浮游动物碳、氮稳定同位素比值的影响[J]. , 2011, 30(2): 195-200.
HAN Long1, LIU Zheng-wen1,2. Effect of pretreatment and preservation procedures on carbon and nitrogen stable isotope ratios of zooplankton. , 2011, 30(2): 195-200.
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