Analysis and improvement of high-quality RNA extraction in leaves of mangrove plants
SONG Hui1,3, WANG You-shao1,2
1. Key Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute of Oceanology; Chinese Academy of Sciences, Guangzhou 510301, China; 2. Marine Biology Research Station at DayaBay, Chinese Academy of Sciences, Shenzhen, 518121, China; 3. Graduate School of the ChineseAcademy of Sciences, Beying, 100039, Chtna
Five extraction methods including Takara RNA,Tiangen kit,Invitrogen kit,modified CTAB-LiCl and CTAB-isopropyl alcohol method were compared for extracting total RNA from the leaves of four mangrove plants(Bruguiera gymnorrhiza,Kandelia candel,Aegiceras corniculatum,and Aricennia marina).The five methods were all suitable for total RNA extraction in leaves of A. marina.Tiangen kit was successfully used to extract the RNA in leaves of A.corniculatum,A.marina,and K.candel,but failed to extract the RNA in leaves of B.gymnorrihiza.Invitrogen kit extracted high quality RNA of 5.gymnorrihiza and K.candel,but could not remove the protein of leaves of A.corniculatum.The RNA amount extracted using CTAB-LiCl and improved CTAB-isopropyl alcohol methods was lower than using RNA extraction kits;besides,the efficiency of reverse transcription PCR of CTAB-LiCl and CTAB-isopropyl alcohol methods was lower.
宋晖1,3*, 王友绍1,2. 红树植物叶片总RNA提取方法比较与优化[J]. , 2011, 30(2): 201-206.
SONG Hui1,3, WANG You-shao1,2. Analysis and improvement of high-quality RNA extraction in leaves of mangrove plants. , 2011, 30(2): 201-206.
[1] Fu X,Deng S,Su G,Zeng Q,Shi S.Isolating high-quality RNA from mangroves without liquid nitrogen[J].Plant Molecular Biology Reporter,2004,22:197a-197e.
[2] Ezawa S,Tada Y Identification of salt tolerance genes from the mangrove plant Brguiera gymnorrhiza using Agrobacterium functional screening[J].Plant Science,2009,176(2):272-278.
[3] Huang G Y,Wang Y S.Expression and charactenzation analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress[J].Aquatic Toxicology,2010,99(1):86-92.
[5] Ghangal R,Raghuvanshi S,Chand Sharma P.Isolation of good quality RNA from a medicinal plant seabuckthom,rich in secondary metabolites[J].Plant Physiology and Biochemistry,47:1113-1115.
