The methanol,ethanol and 90% acetone were used to extract seven strains of Eustigmatophyceae pigments respectively.Extracting efficiency of three kinds of organic solvents on pigments was detected,the absorption spectrum of three organic solvent pigment extractions was measured,then,content of chlorophyll a and carotenoid were evaluated with spectrophotometric method.Moreover,the maximum absorption peaks of methanol pigment extraction and ethanol pigment extraction on A470 and A666 was compared.The results showed: extracting with ethanol had higher efficiency while lower toxicity compared with methanol and 90% acetone.Content of chlorophyll a and carotenoid extracted by three kinds of organic solvent had no significant differences,while extracting rates in three kinds of organic solvent was similar(P>0.05).Also,the absorption spectrum of pigments extracted by three kinds of organic solvent were similar,and the maximum absorption peaks of methanol pigment extraction and ethanol pigment extractionin at 666 nm and 470 nm showed no significant differences(P>0.05).Content of pigment extracted by ethanol could be calculated by the Lichtenthaler equation.
李其雨, 李爱芬*, 张成武. 真眼点藻类色素的提取与测定方法[J]. , 2012, 31(3): 278-283.
LI Qi-yu, LI Ai-fen, ZHANG Cheng-wu. The method of extraction and determination of pigment in Eustigmatophyceae. , 2012, 31(3): 278-283.
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