Growth dynamic characteristics of Phaeocystis globosa,a representative HAB species,as a function of six different nitrogen sources: nitrate,urea,glycine,arginine,glutamic acid and ATP,were examined in batch cultures.The laboratory results revealed that P.globosa could utilize all the six nitrogen sources,and exhibited obvious concentration gradient effect.The maxium specific growth rates of P.globosa under different nitrogen sources were fitted with Monod Kinetics,the result showed the maxium specific growth rates with nitrate,urea,glycine,arginine,glutamic acid,reached 1.05,1.17,0.82,0.87,1.09,0.90 d-1 respectively,and the corresponding half saturation constants were 9.132,23.758,85.519,7.104,23.94,10.959 μmol/L respectively.In comparison,Growth inhibition was observed in ATP culture with the highest nitrogen gradient 8 820 μmol/L,P.globosa showed the greatest affinity to glycine.The maximum photosynthetic efficiency reached 0.619,0.620,0.579,0.595,0.648,0.667 respectively when the nitrogen gradient were 8 820,882,882,8 820,882,0.441 μmol/L of different N-sources.These results suggested that the growth of P.globosa varies significantly with different N-sources,P.globosa could utilize various kinds of inorganic and organic nitrogen sources,thus providing P.globosa with advantage comparing to other algae utilizing dissolved inorganic nitrogen as the sole nitrogen source.
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