Effects of elevated ozone concentration on photosynthetic pigment and resistance physiology in leaves of Phyllostachys edulis
ZHUANG Ming-hao1, LI Ying-chun1, GUO Zi-wu1, YANG Qing-ping1, CHEN Shuang-lin1, LI Ying1,2, GU Li-jian3
1. Research Institute of Subtropical Forest, Chinese Forestry Academy, Fuyang 311400, China; 2. Institute of Forest, Guizhou University, Guiyang 550025, China; 3. Hanzhou Lin'an Taihuyuan Landscaping Bamboo Garden, Lin'an 311300, China
In order to explore the management strategies of bamboo under the context of climate change,the effects of elevated ozone concentrations on photosynthetic pigments content,soluble protein content,lipid peroxidation and anti-oxidation system in the leaves of Phyllostachys edulis grown in open-top chambers(OTCs) were investigated.The results showed that with the ozone concentration increasing,the contents of the chlorophyll,carotenoid,and SOD activity decreased;while the contents of soluble protein,MDA,O2- and POD activity increased.Elevated ozone concentrations at 100 and 150 nl·L-1 significantly decreased the photosynthetic pigments content and SOD activity;while increased soluble protein content,MDA content,O2- content and POD activity.The results suggested that the elongated ozone exposure inhibited the growth of P.edulis by restraining the synthesis or speeding the degradation of photosynthetic pigments,accelerating the aging of leaves,intensifying the damage to membrane lipid,and destroying the structure of cell membrane and the function of anti-oxidation system.
[1] Prenticer I,Arquhar G,Fasham M.The carbon cycle and atmospheric carbon dioxide in climate change 2001[C].Cambridge.UK:Cambridge University Press,2001.
[2] Tausz M,Grulke N E,Wieser A.Defense and avoidance of ozone under global change[J].Environment Pollution,2007,147:525-531.
[3] 张宪政,陈凤玉.植物生理学试验技术[M].辽宁:辽宁科技出版社,1994.164-165.
[4] 李忠光,龚明.植物中超氧阴离子自由基测定方法的改进[J].云南植物研究,2005,27(2):211-216.
[5] 陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2006.54-124.
[6] Castagna A,Nali C,Ciompi S,Lorenzini G,Soldatini G F,Ranieri A.Ozone exposure affects photosynthesis of pumpkin(Cucurb ita pepo) plants[J].New Phytologist,2001,152(2):223-229.
[7] Riikka Rinnan,Toini Holopainen.Ozone effects on the ultrastmcture of peatland plants:Sphagnum mosses,Vaccinium oxycoccus,Andromeda polifolia and Eriophorum vaginatum[J].Annals of Botany,2004,94(4):623-634.
[8] Yah K,Chen W,He X Y,Zhang G Y,Xu S,Wang L L.Responses of photosynthesis,lipid peroxidation and antioxidant system in leaves of Quercus mongolica to elevated O3[J].Environment and Experimental Botany,2010,69:198-204.
[9] Susana E,Roeio A,Federico J C,Gimeno B S.On the response of pigments and antioxidants of pinus halepensis seedlings to Mediterranean climatic factors and long-term ozone exposure[J].New Phytologist,1998,138:419-432.
[10] Wustman B A,Oksanen E,Kamosky D F,Noormets A,Isebrands J G,Pregitzer K S,Hendrey G R,Sober J,Podila G K.Effects of elevated CO2 and O3 on aspen clones varying in O3 sensitivity:can CO2 ameliorate the harmful effects of O3[J].Environmental Pollution,2001,115:473-481.
[11] Anil S,Grantz D A.Ozone impacts on competition between tomato and yellow nutsedge:Above-and below-ground effects[J].Crop Science,2005,45(4):1587-1595.
[12] 付宇.不同臭氧浓度对大豆活性氧代谢及保护同工酶的影响[D].沈阳:沈阳农业大学农学院,2009.
[13] Neill S J,Desikan R,Clarke A.Hydrogen peroxide and nitric oxide as signaling molecules in plant[J].Journal of Experimental Botany,2002,53:1237-1247.
[14] 金明红,冯宗炜,张福珠.臭氧对水稻叶片膜脂过氧化和抗氧化系统的影响[J].环境科学,2000,21(3):3-5.
[15] 杨铁钊,殷全玉,丁永乐,张玉梅.烟草气孔特性、抗氧化酶活性与臭氧伤害的关系[J].植物生态学报,2004,28(5):672-679.
[16] Tandy N E,Ginlio R T,Richardson C J.Assay and electrophoresis of superoxide dismutase from red spruce(Picea rubens Sarg),loblolly pine(Pinus taeda L.),and Scotch pine(Pinus Sylvestris L.):A method for biomonitoring[J].Plant Physiology,1989,90:742-748.
[17] He X Y,Ruan Y N,Chen W,Lu T.Responses of antioxidative system in leaves of Ginkgo biloba to elevated ozone concentration in urban area[J].Botanical Studies,2006,47:409-416.
[18] Robinson D C,Wellbum A R.Seasonal changes in the pigments of Norway spruce(Picea abies.L)Karst,and the influence of summer ozone exposure[J].New Phytologist,1991,119:251-259.
[19] 金明红,冯宗炜.臭氧对冬小麦叶片膜保护系统的影响[J].生态学报,2000,20(4):444-447.
[20] Zhao T H,Shi Y,Huang G H,Wang Y,Sun B.Respective and interactive effects of double CO2 and O3 concentrations on membrane lipid peroxidation and antioxidative ability of soybean[J].Science in china(Series C Life Science),2005,48:136-141.
[21] Zhao T H,Wang J L,Wang Y,Sun J W,Cao Y.Effects of reactive oxygen species metabolic on soybean(Glycine max)under exogenous chitosan to ozone stress[J].Bull Environ Contam Toxicol,2010,85:59-63.
[22] Gillespie K M,Rogers A,Ainsworth E A.Growth at elevated ozone or elevated carbon dioxide concentration alters antioxidant capacity and response to acute oxidative stress in soybean(Glycine max)[J].Journal of Experimental Botany,2011,62:1-12
[23] 卢涛,何兴元,陈炜.O3和CO2浓度升高对油松针叶抗氧化系统的影响[J].生态学杂志,2009,28(7):1316-1323.
[24] Vancamp W,Willekens H,Bowler C,Montagu M V,Inze D,Reupold-Popp P,Sandermaun H,Langebartels J C.Elevated levels of superoxide dismutase protect transgenic plants against ozone damage[J].Nature Biotechnology,1994,12:165-168.
[25] Casano L M,Gomez L D,Lascan H R,Gonzalez C A,Trippi V S.Inactivation and degradation of CuZn-SOD by active oxygen species in wheat chloroplasts exposed to photo oxidative stress[J].Plant and Cell Physiology,1997,38:433-440.
[26] Bhattacharya J,Ghoshdastidar K,Chatterjee A,Majee M,Majumder A L.Synechocystis Fe superoxide dismutase gene confers oxidative stress tolerance to Escherichia coli[J].Biochemical and Biophysical Research Communications,2004,316:540-54.
[27] Lu T,He X Y,Chen W,Yan K,Zhao T H.Effects of elevated O3 and/or elevated CO2 on lipid peroxidation and anantioxidant systems in Ginkgo biloba leaves[J].Bull Environ Contam Toxicol,2009,83:92-96
[28] Yan K,Chen W,Zhang G Y,Xu S,Liu Z L,He X Y,Wang L L.Elevated CO2 ameliorated oxidative stress induced by elevated O3 in Quercus mongolica[J].Acta Pyhsiologiae Plant,2010,32:375-385
[29] Asada K.Ascorbate peroxidase:a hydrogen peroxide-scavenging enzyme in plant[J].Physilogia Plantarum,1992,85:235-241.
[30] Matters G L,Scandalios J G.Synthesis of isozymes of superoxide dismutase in maize leaves in response toO3,SO2 and elevated O2[J].Journal of Experimental Botany,1986,38:842-852.
[31] 张巍巍,郑飞翔,王效科,冯兆忠,欧阳志云,冯宗炜.大气臭氧浓度升高对对水稻叶片膜脂过氧化及保护酶活性的影响[J].应用生态学报,2008,19(11):2485-2489.
[32] 黄玉源,黄益宗,李秋霞,张施君,唐桂芬,林家华.臭氧对南方三种木本植物的急性伤害症状及其生理指标变化[J].生态环境,2006,15(4):674-681.
