短尾猴与红面猴细胞色素b基因变异特点及系统发育分析

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摘要

测定了2个短尾猴和1个红面猴个体的线粒体细胞色素b基因全序列,与猕猴、食蟹猴和叟猴的序列合并比较,分析了核苷酸序列差异和碱基替换特点,并以阿拉伯狒狒为外群,构建系统发生树。结果表明,短尾猴基因序列中A、T、G、C的含量分别为29.0%、25.5%、11.8%、33.6%,红面猴中A、T、G、C的含量分别为29.4%、24.3%、11.4%、34.9%,碱基组成具有哺乳动物的共同特点,短尾猴与红面猴序列间的同源性为90.1%。红面猴与食蟹猴之间的同义替换和异义替换值(Ks、Ka)以及Kimura双参数距离都要小于红面猴与短尾猴之间的差异值,在构建的系统发生树中,叟猴最早分化出来,红面猴并未与短尾猴聚为一支,而是与猕猴、食蟹猴聚在了一起。

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	柳杨
	李进华
	赵健元

关键词 ：短尾猴, 红面猴, 细胞色素b, 序列变异, 系统发育

Abstract：

The complete mitochondrial cytochrome b genes were sequenced from two individuals of Tibetan macaque (Macaca thibetana) and one individual of stump-tailed macaque(Macaca arctoides).Through comparison with three other macaque species, the base composition and sequence divergence among these sequences were analyzed.The phylogenetic tree was constructed with Papio hamadryas as the outgroup.The contents of A、T、G、C were 29.0%、25.5%、11.8%、33.6% and 29.4%、24.3%、11.4%、34.9% in the cyt b gene sequences of the Tibetan macaque and stump-tailed macaque,respectively.The base composition of the Tibetan macaque and stump-tailed macaque showed the same character as that of other mammals.There was 90.1% homology with 113 variant sites between the two sequences.Results showed apparent divergence between Tibetan macaque and stump-tailed macaque.The phylogenetic tree supported the topology that Macaca sylvanu diverged firstly from other macaque species,and Macaca arctoides gathered together with M.mulatta and M.fascicularis rather than with M.thibetana.

Key words： Macaca thibetana Macaca arctoides Cytochrome b Sequence divergence Phylogeny

收稿日期: 2006-10-25

Q951

基金资助:国家自然科学基金(39970109,30570237);教育部骨干教师资助计划(18557);安徽省优秀青年基金(04043070);安徽省教育厅自然科学基金(2005KJ021ZD);安徽大学学术创新团队项目

引用本文:

柳杨1,2, 李进华1,2*, 赵健元2,3. 短尾猴与红面猴细胞色素b基因变异特点及系统发育分析[J]. , 2006, 25(5): 426-429.
LIU Yang1,2, LI Jin-hua1,2, ZHAO Jian-yuan2,3. Divergence and phylogeny of mitochondrial cytochrome b gene from Tibetan macaque and stump-tailed macaque. , 2006, 25(5): 426-429.

链接本文:

http://www.ecolsci.com/CN/Y2006/V25/I5/426

[1] Allen G M.1938.The mammals of China and Mongolia,part Ⅰ[M].New York:American Museum of Natural History Press.273-279.

[2] Fooden J.1976.Provisional classifications and key to living species of macaques (Primates:Macaca)[J].Folia Primatologica,25:225-236.

[3] Fooden J.1983.Taxonomy and evolution of the sinica group of macaques:4.Species account of Macaca thibetana[J].Fieldiana Zool.,17:1-20.

[4] Delson E.1980.Fossil macaques,phyletic relationships and a scenario of development.In The Macaques:Studies in Ecology,Behavior,and Evolution[M].New York:Van Nostrand Reinhold,10-30.

[5] Fooden J,Lanyon S M.1989.Blood-protein allele frequencies and phylogenetic relationships in Macaca:a review[J].American Journal of Primatology,17:209-241.

[6] 王文,宿兵,兰宏,等.1996.猕猴属6个种的rDNA变异及其系统进化关系[J].遗传学报,23:343-350.

[7] Zhang Y P,Shi L M.1993.Phylogenetic relationships of macaques as inferred from restriction endonuclease analysis of mitochondrial DNA[J].Folia primatologica,60:7-17.

[8] Hayasaka K,Fujii K,Horai S.1996.Molecular phylogeny of macaques:implications of nucleotide sequences from an 896-base pair region of mitochondrial DNA[J].Molecular Biology and Evolution,13:1044-1053.

[9] Irwin D M,Kocher T D,Wilson A C.1991.Evolution of the Cytochrome b Gene of Mammals[J].Journal of Molecular Evolution 32:128-144.

[10] 曹祥荣,束峰珏,张锡然,等.2002.毛冠鹿与3种麂鹿动物的线粒体细胞色素b的系统进化分析[J].动物学报,48(1):44-49.

[11] 曹丽荣,王小明,饶刚,等.2004.从细胞色素b基因全序列分析岩羊和山羊、绵羊的系统发生关系[J].兽类学报,24(2):109-114.

[12] 吕雪梅,王应祥,张亚平.2001.蜂猴线粒体细胞色素b基因变异特点及系统发育分析[J].动物学研究,22(2):93-98

[13] Higuchi R,Beroldingen von C H,Sensabaugh G F,et al.1988.DNA typing from single hairs[J].Nature,332:543-546.

[14] Kocher T D,Thomas W K,Meyer A,et al.1989.Dynamics of mitochondrial DNA evolution in animals:amplification and sequencing with conserved primers[J].Proceedings of the National Academy of Sciences of the United States of America,86:6196-6200.

[15] Jeanmougin F,Thompson J D,Gouy M,et al.1998.Multiple sequence alignment with Clustal[J].Trends Biochem.Sci,23:403-405.

[16] Kumar S,Tamura K,Jakobsen B I,et al.2001.Molecular evolutionary genetics analysis,ver 2.1[J].Bioinformatics,17 (12):1244-1245.

[17] Xia X,Xie Z.2001.Data analysis in molecular biology and evolution[J].Journal of Heredity,92:371-373.

[18] Li W H.1993.Unbiased estimation of the rates of synonymous and nonsynonymous substitution[J].Journal of Molecular Evolution,36:96-99.