Application study of high-efficiency bioflocculant D6 in slaughter wastewater
FU Bao-rong1, LIU Dong1, QIAO Yong-min2, CHEN Shi2, XU Cheng-bin1, MA Xi-ping1, HUI Xiu-juan1
1. School of Environmental Science, Liaoning University, Shenyang 110036, China; 2. College of Life Science and Technology, Jinan University, Guangzhou 510632, China
One bioflocculant-producing strain of high flocculating activity was screened for slaughter wastewater.The optimum flocculating conditions of bioflocculant D6 produced from this strain were obtained by single factor test and orthogonal design test.Flocculating conditions included bioflocculant D6 dosage,pH,kinds of metal ions and 1% CaCl2 dosage.The study found bioflocculant D6 had flocculating activity under precondition of Alkaline environment,it indicated full extension of molecular chains of bioflocculant D6 was the determinant for flocculation,so the flocculating mechanism of bioflocculant D6 was adsorption-bridge.The optimum flocculating conditions were as follows:bioflocculant D6 dosage 25 mL·L-1,1%CaCl2 dosage 55 mL·L-1,pH 8.In the optimum flocculating conditions,flocculation rate was 96.6%,turbidity removal rate was 97.8%,SS removal rate was 92.6%.
[1] Yokoi H.Flocculation properties of poly (gamma-glutamic acid) produced by bacillus subtilis[J].Journal of Fermentation and Bioengineering,1996,8(1):84-87.
[2] Suh H H.Characterization of bioflocculant produced by bacillus sp.DP-152[J].Journal of Fermentation and Bioengineering,1997,84 (2):108-112.
[3] Uma B,Sandhya S.Pyridine degradation and heterocyclic nitrification by Bacillus coagulans[J].Canadian Journal of Microbiology,1997,43(6):595-598.
[4] Lee S H,Lee S O,Jang K L,Lee T H.Microbial flocculant from Arcuadendron sp TS-49[J].Biotechnology Letter,1995,17 (1):95-100.
[5] MA Fang,WANG Bo,FAN Chun,YANG Ji-xian,LI Bai-xiang,LIU Yan-bin.Security evaluation of compounded microbial flocculant[J].Journal of Harbin Institute of Technology,2004,11(4).
[6] 康茹.BFA_60微生物絮凝剂絮凝效果实验研究[J].安全与环境学报,2004,4(5):84-85.
[7] 胡勇有,高宝玉.微生物絮凝剂[M].北京:化学工业出版社.2007.
[8] 常青.水处理絮凝学[M].北京:化学工业出版社.2003.
[9] 严丽君,沈彩虹,丁国际,施嘉明,华雯茹,朱莹莹.微生物絮凝剂对微颗粒高岭土悬浊液的絮凝特性[J].海洋环境科学,2009,28(4):433-437.
[10] 王兰.生物絮凝剂絮凝活性评价方法的探讨[J].生物技术,2005,15(5):57-59.