Analysis on bacterioplankton community structure in two oligotrophic lakes, Huashen Lake and Zixia Lake
BIAN Yuan-qi1,2, XING Peng1, LI Hua-bing1,2, WU Qing-long1
1. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100039, China
In order to explore the composition of bacterioplankton community in oligotrophic lakes, clone libraries were constructed and analyzed for Huashen Lake and Zixia Lake located in the urban area of Nanjing City, respectively. Results showed that positive clones of Huashen Lake and Zixia Lake were mainly affiliated with Actinobacteria, Cyanobacteria, Alphaproteobacteria, Betaproteobacteria, Bacteroidetes, Planctomycetes, Verrucomicrobia and Gemmatimonadetes. About 75% of total bacterial clones obtained from Huashen Lake and Zixia Lake were higher than 97% of homology with uncultured bacteria in GenBank. Meanwhile, six new freshwater bacterial clusters were found simultaneously in Huashen Lake clone library and Zixia Lake clone library. The present study has broadened our knowledge on bacterioplankton diversity and suggested that the diversity of bacterioplankton in freshwater lakes still needs further study.
边园琦1,2*, 邢鹏1, 李化炳1,2, 吴庆龙1. 贫营养湖泊花神湖和紫霞湖浮游细菌群落结构分析[J]. , 2010, 29(2): 109-115.
BIAN Yuan-qi1,2, XING Peng1, LI Hua-bing1,2, WU Qing-long1. Analysis on bacterioplankton community structure in two oligotrophic lakes, Huashen Lake and Zixia Lake. , 2010, 29(2): 109-115.
[1] Raymond P A, Bauer J E.Bacterial consumption of DOC during transport through a temperate estuary[J].Aquatic Microbial Ecology, 2000, 22:1-12
[2] Zwart G, Crump B C, Agterveld M P K-V, et al.Typical freshwater bacteria:an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers[J].Aquatic Microbial Ecology, 2002, 28:141-155.
[3] Xing P, Hahn M W, Wu Q L.Low Taxon Richness of Bacterioplankton in High-Altitude Lakes of the Eastern Tibetan Plateau, with a Predominance of Bacteroidetesand Synechococcus spp.[J].Applied and Environmental Microbiology, 2009, 75(22):7017-7025.
[4] 金相灿, 屠清瑛.湖泊富营养化调查规范[M].第二版.北京:中国科学出版社.1990.160-184.
[5] Gucht K V d, Cottenie K, Muylaert K, et al.The power of species sorting:Local factors drive bacterial community composition over a wide range of spatial scales[J].PNAS, 2007, 104(51):20404-20409.
[6] Hill T C J, Walsh K A, Harris J A, et al.Using ecological diversity measures with bacterial communities[J].FEMS Microbiology Ecology, 2003, 43:1-11.
[7] Hughes J B, Hellmann J J, Ricketts T H, et al.Counting the Uncountable:Statistical Approaches to Estimating Microbial Diversity[J].Applied and Environmental Microbiology, 2001, 67(10):4399-4406.
[8] Lindstro m E S, Agterveld M P K-V, Zwart G Distribution of Typical Freshwater Bacterial Groups Is Associated with pH, Temperature and Lake Water Retention Time[J].Applied and Environmental Microbiology, 2005, 71(12):8201-8206.
[9] Wu Q L, Chen Y W, Xu K D, et al.Intra-habitat heterogeneity of microbial food web structure under the regime of eutrophication and sediment resuspension in the large subtropical shallow Lake Taihu, China[J].Hydrobiologia, 2007, 581:241-254.
[10] Hahn M W, Pckl M.Ecotypes of planktonic Actinobacteria with identical 16S rRNA genes adapted to thermal niches in temperate, subtropical and tropical freshwater habitats[J].Applied and Environmental Microbiology, 2005, 71:766-773.