Since phenol chemical compound is permanent organic pollutant, phenol-containing wastewater treating has the high priority in water pollution control in our country.In this paper, bacteria strains were separated and purified from the active mud of a ventilated pool in which wastewater containing phenol was treated.7 bacteria strains that could treat phenol-containing wastewater with high efficiency were chosen according to their phenol tolerance and phenol degradation rate.Based on the microscopic observation of the characteristics of colony and individual cell, and the results of a series of physiology and biochemical tests, those 7 chosen bacteria strains were identified to be Pseudomonas.This work is beneficial for the phenol-containing wastewater treatment in the future.