In order to develop a method for counting the number of seawater fungi using culture medium flat, the samples were collected by different sampling methods with sterile syringes, organic glass hydrophore, and (then) were diluted by two liquids-seawater and freshwater-and incubated by five culture mediums-Potato agar (PA), Potato dextrose agar (PDA), Malt extract media (MA), Czapek-dox agar (CA) and Yeast extract peptone dextrose agar (YPD). Each medium was blended with seawater and freshwater, respectively. Results showed that 1) the amount of fungi in the same medium had no obvious difference with different sampling method; 2) there were hardly any fungi in PDA and PA mediums blended with seawater; 3) a few mildews developed in MA medium without obvious differences among the disposals of different liquids in sample dilutions or medium blend; and 4) the populations of fungi-mainly yeasts-in YPD and CA mediums showed differences among the different disposals, with the highest fungal loads observed in seawater YPD medium when seawater sample was diluted by freshwater. Thus, it demonstrated that the seawater YPD with sample diluted by freshwater was more suitable for counting fungi in seawater than other treatments in the present study.
朱红, 许忠能, 林小涛*, 孙军. 海水真菌定量分析方法的初步研究[J]. , 2007, 26(2): 155-158.
ZHU Hong, XU Zhong-neng, LIN Xiao-tao, SUN Jun. Preliminary study on the quantitative analysis of marine fungi by plate count method. , 2007, 26(2): 155-158.
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