To obtain hybridoma cell line which can secrete anti-gonyautoxin 2,3(GTX2,3) monoclonal antibodies(mAb), the immunogen(GTX2,3-BSA) and solid phase immunogen(GTX2,3-KLH) were prepared by the method of aldehyde conjugation, in which GTX 2,3 was conjugated with BSA and KLH respectively. The spleen cells from BALB/c mice immunized with GTX2,3-BSA were fused with murine Sp2/0 myeloma cells in PEG4000. The specific hybridoma cells and the ascites were analyzed by ELISA. Three specific hybridoma cell lines which can secrete anti-GTX 2,3 mAb were obtained. The titer of the mAb in the ascites was about 1.4×10-5. The specific anti-GTX2,3 mAb can be obtained from hybridoma successfully, which can used to detect the PSP from seafood.
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