大孔树脂提取链霉菌4903菌株除草活性物质的研究

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摘要

化感活性物质的提取方法是研究化感作用的重要步骤,对化感链霉菌4903的前期研究证明其具有抑菌及除草活性。本文选用了4种大孔吸附树脂(X-5, NKA-II, AB-8和HP-20)对化感链霉菌4903发酵液的除草活性物质进行提取。实验结果显示:树脂AB-8对链霉菌4903菌株发酵液除草活性成分具有较好的吸附性能和较易被解吸的特性,树脂AB-8对链霉菌4903菌株发酵液的静态吸附量约可达到树脂体积的7倍。用树脂2-3倍体积的80%乙醇溶液则可以把吸附的除草活性物质全部从树脂中解吸出来。AB-8适合用于链霉菌4903菌株发酵液除草活性物质的提取和纯化。

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	陈敏
	王瑞龙
	曾任森

关键词 ：大孔吸附树脂, 除草活性, AB-8 树脂, 链霉菌4903

Abstract：

Isolation of allelochemicals is an important step to study allelopathy. Our previous work demonstrated that Streptomyces strain 4903 showed allelopathic effects on both plants and plant fungal pathogens. In this study four macroporous resins (X-5, NKA-II, AB-8 and HP-20) were used to collect herbicidal substances from fermentation broth of Streptomyces strain 4903. Among the four resins, AB-8 macroporous resin had the highest adsorption capacity for herbicidal compounds from the fermentation broth. The static saturation adsorption capacity was 7 times that of resin volume. The optimal eluant eluted herbicidal compounds of Streptomyces strain 4903 from AB-8 resin was 80% (v/v) ethanol–water. All herbicidal compounds of Streptomyces strain 4903 were able to be desorbed by 2-3 times that of its resin volume. The AB-8 resin can be used for extraction and purification of herbicidal compounds from fermentation broth of Streptomyces strain 4903.

Key words： macroporous adsorption resin herbicidal substances AB-8 resin Streptomyces strain 4903

收稿日期: 2013-08-25

TQ424.3

基金资助:国家自然科学基金项目(21102049);广东省科技计划项目(2009B080701047)

引用本文:

陈敏, 王瑞龙, 曾任森*. 大孔树脂提取链霉菌4903菌株除草活性物质的研究[J]. , 2013, 32(4): 469-473.
Chen Min, WANG Rui-long, Zeng Ren-sen*. Study on extraction of herbicidal substances from Streptomyces sp. 4903 with macroporous resins. , 2013, 32(4): 469-473.

链接本文:

http://www.ecolsci.com/CN/Y2013/V32/I4/469

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