Studies on activities of extracellular enzymes of four yeasts and their degradation of organic pollutants from aquaculture
DAI Shun-zhen, XIA Xin-jian, XU Zhong-neng, LIN Xiao-tao*
Institute of Hydrobiology, Jinan University, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institutes, Guangzhou 510632, China
Production of amylase, protease, lipase, and cellulose of four marine yeasts was compared using differential medium plate method, and the enzyme activities were also analyzed in this research. The results showed that Candida colliculosa DY11-1 could secrete amylase, protease and lipase, and their activities were 181.4, 123.1 U/mL and 54.2 U/mL, respectively. Pichia sp DY11-6 and Candida parapsilosis DY07-1 could secrete amylase and protease. Their amylase activities were 108.1 and 96.5 U/mL, and their protease activities were 35.1 and 128.4 U/mL, respectively. Rhodotorula glutinis DY02-3 did not secrete the four extracellular enzymes. Feed extracts of Tilapia mossambica were used as aquaculture organic pollutants to compare the degradation ability of the four marine yeasts. The results showed that Candida colliculosa DY11-1 possessed the strongest degradation capacity. The concentration of COD, TN and TP in the tested water decreased from 27.60, 12.22 and 2.67 mg/mL to 15.73, 7.59 and 1.40 mg/mL after 9 days, respectively, with the degradation rates of 43.0%, 37.8% and 47.5%. It indicated that DY-11 had potential application value in improving water quality of aquaculture.
戴顺珍, 夏新建, 许忠能, 林小涛*. 4株海洋酵母菌胞外酶活力及其对水产养殖有机污染物的降解[J]. , 2013, 32(1): 22-26.
DAI Shun-zhen, XIA Xin-jian, XU Zhong-neng, LIN Xiao-tao*. Studies on activities of extracellular enzymes of four yeasts and their degradation of organic pollutants from aquaculture. , 2013, 32(1): 22-26.
[1] 孙舰军, 丁美丽. 氨氮对中国虾抗病力的影响[J]. 海洋与湖泊, 1999, 30(3):367-372.
[2] Chinni S, Khan R N, Yallapragada P R. Acute toxicity of lead on tolerance, oxygen consumption, ammonia-N excretion, and metal accumulation in Penaeus indicus postlarvae[J]. Ecotoxicol Environ Saf, 2002, 51(2): 79-84.
[3] 秦伯强, 胡维平, 刘正文. 太湖水源地水质净化的生态工程试验研究[J]. 环境科学学报, 2007, 27(1):5-12.
[4] 丁彦文, 艾红. 微生物在水产养殖中的应用[J]. 湛江海洋大学学报, 2000, 20(3):68-73.
[5] 宫清松. 微生物制剂在水产养殖中应用浅析[J]. 中国水产, 2003, (6):83-84.
[6] 王华, 陈有容. 益生菌和水产动物饲料添加剂[J]. 中国微生态学杂志, 2001, 13(3):181-183.
[7] 仇丽. 枯草芽孢杆菌在养殖中的应用[J]. 渔业现代化, 2002, (4):26.
[8] 池振明, 龚方, 李静等. 海洋酵母菌胞外酶及其基因的最新研究进展[J]. 中国海洋大学学报(自然科学版), 2008, 38(5):766-744.
[9] 夏霄鹤. 海洋红酵母-健康的微生态活菌制剂[J]. 中国校外教育, 2009, (09):49.
[10] 吴伟, 余晓丽, 李咏梅. 假丝酵母对养殖水体中亚硝酸盐的降解特性[J]. 中国环境科学, 2001, 21(1):8-11.
[11] 谢航, 邱宏端, 林娟等. 假丝酵母菌降解养殖水体氨氮的特性研究[J]. 农业工程学报, 2005, 21(8):142-145.
[12] Li H F, Chi Z M. Amylase production by the Marine Yeast Aureobasidium pullulans N13d[J]. Journal of Ocean University of China, 2007, 6(1): 60-65.
[13] 王晓红, 池振明. 产淀粉酶海洋酵母菌的筛选及鉴定[J]. 中国海洋大学学报(自然科学版), 2007, 37(6):95-100.
[14] Chi Z M, Liu Z Q, Gao L M, et al. Marine yeasts and their applications in mariculture[J]. Journal of Ocean University of China, 2006, 5(3): 251-256.
[15] Kudanga T, M wenje E. Extracellular cellulase production by tropical isolates of 479 Aureobasidium pullulans[J]. Microbiol, 2005, 51: 773-776.
[16] 林东年, 叶宁, 刘兴华, 黄勇安, 张亚宇. 光合细菌对罗非鱼塘中水质和生态的影响[J]. 水土保持研究, 2007, 14(2):207-212.
[17] 胡菊香, 吴生桂, 邹清, 陈金生, 胡小健, 胡传林. 生物水净化剂对养殖池塘水质的调控作用初探[J]. 水利渔业, 2003, 23(6):40-41.
[18] Fei Liu,Wu Yinghan. Reuse strategy of wastewater in prawn nursery by microbialremediation[J]. Aquaculture, 2004, 230(5): 281-296.
