生长阶段及环境因子对东海原甲藻氨基酸氧化酶活性的影响

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摘要

以中国沿海典型赤潮藻东海原甲藻为实验材料, 研究了不同生长阶段以及温度、光照和氮源对其氨基酸氧化酶活性的影响。结果表明, 在缺氮条件下东海原甲藻显示较高的氨基酸氧化酶活性。在15-30℃温度范围均检测出氨基酸氧化酶活性, 较低温度下(15-20℃)的氨基酸氧化酶活性显著高于高温处理组(25-30℃)(p<0.05), 其中20℃时的酶活最高。在50-100 μmol/(m²·s)的光强下, 氨基酸氧化酶活性较高(0.38-0.47 fmol/(h·cell)), 而在2 μmol/(m²·s)的低光强下, 虽然酶活受到显著抑制, 但仍达到0.28 fmol/(h·cell)。氮源组成对氨基酸氧化酶活性具有显著影响, 以丙氨酸为唯一氮源时的酶活最高(0.44 fmol/(h·cell)), NH₄⁺+丙氨酸为氮源时的酶活最低(0.22 fmol/(h·cell))。研究显示, 光照、温度和氮源是东海原甲藻氨基酸氧化酶活性的关键调控因子。东海原甲藻不仅能够有效利用游离氨基酸, 而且适应较广的温度和较低的光照条件, 这可能是东海原甲藻赤潮形成和持续的重要原因。

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	刘静雅
	赖海燕
	徐宁
	段舜山

关键词 ：东海原甲藻, 溶解游离氨基酸, 可溶性有机氮, 氨基酸氧化酶, 环境因子

Abstract：

Effects of growth phase, temperature, irradiance and nitrogen source on the amino acid oxidase (AO) activity of Prorocentrum donghaiense, a causative organism of large-scale harmful algal blooms (HABs) along the coastal waters of East China Sea, were investigated in the laboratory. The results showed that P. donghaiense displayed higher amino acid oxidase activity in N-depleted conditions. AO activity of P. donghaiense was detected at the temperatures ranging from 15 to 30℃, in which AO activity was significantly higher at lower temperatures (15℃-20℃) than that at higher temperatures(25℃-30℃) (p<0.05), and the maximum value was observed at 20℃. Although AO activity was relatively higher (0.38 to 0.47 fmol/(h·cell)) at higher irradiances (50-100 μmol/(m²·s)), AO activity was still up to 0.28 fmol/(h·cell) at irradiance of 2 μmol/(m²·s¹). Nitrogen source exhibited a significant impact on AO activity of P. donghaiense. The highest AO activity (0.44 fmol/(h·cell)) was observed when alanine was used as the sole nitrogen source, and the lowest AO activity (0.22 fmol/(h·cell)) was recorded in the combination of ammonium and alanine. It suggested that temperature, irradiance and nitrogen source were key environmental factors regulating AO activity of P. donghaiense. P. donghaiense is not only capable of utilizing free amino acids effectively, but also flexible in adapting to broad temperature and lower irradiance, which may play important roles in the formation and duration of P. donghaiense blooms.

Key words： Prorocentrum donghaiense dissolved free amino acid (DFAA) dissolved organic nitrogen (DON) amino acid oxidase (AO) environmental factor

收稿日期: 2013-02-25

Q178.1

基金资助:国家自然科学基金项目(40776078, 41176104, U1133003)资助

引用本文:

刘静雅, 赖海燕, 徐宁*, 段舜山. 生长阶段及环境因子对东海原甲藻氨基酸氧化酶活性的影响[J]. , 2013, 32(1): 27-33.
LIU Jing-ya, LAI Hai-yan, XU Ning*, DUAN Shun-shan. Effects of growth phase and environmental factors on amino acid oxidase activity of Prorocentrum donghaiense. , 2013, 32(1): 27-33.

链接本文:

http://www.ecolsci.com/CN/Y2013/V32/I1/27

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