We conducted a 21-day chronic test with six concentration in order to study the effects of different concentrations of TiO2 nanoparticle on the growth and reproduction of Daphnia magna. The results showed that at the concentration of TiO2 < 1 mg·L-1, adverse effects on the growth and reproduction of Daphnia magna were not significant. At the concentration of TiO2 >1 mg·L-1, adverse effects were significant. As the concentration increased, the survival and reproduction were inhibited obviously. The days to first pregnancy, the days to first breeding, the number of broods per female, number of first broods per female, and the number of offsprings per female were all reduced significantly(p<0.05). At concentration of 5 mg·L-1, breeding was basically stopped, and the intrinsic rate of natural increase (rm) was dropped from 0.347 to 0.106 (control). The 21 d LC50 was 4.16 mg·L-1. The 24 h bioaccumulation experiment indicated that the greater the concentration and time, the higher the body burden. At both low concentration (2 mg·L-1) and high concentration (10 mg·L-1), the burden reached the maximum at 24 h. The 72 h depuration test suggested that the depuration was affected by the concentrations of accumulation. At low concentration (2 mg·L-1), the depuration was stronger than at high concentration (10 mg·L-1), and the biological halftime(T1/2) was 63.3 h and 181.9 h, respectively.
付保荣, 张楠, 武暕*, 李楠, 张润洁, 惠秀娟, 李霞. 不同浓度纳米二氧化钛对大型溞的繁殖及富集和自净能力的影响[J]. , 2012, 31(6): 601-605.
FU Bao-rong, ZHANG Nan, WU Jian*, LI Nan, ZHANG Run-jie, HUI Xiu-juan, LI Xia. Effects of different concentrations of TiO2 nanoparticle on the reproduction, and bioaccumulation and depuration of Daphnia magna. , 2012, 31(6): 601-605.