Effect of transgenic sugarcane on microbial community diversity in rhizosphere soil
TAN Yan-hua1,2, PENG Cun-zhi1,2, ZHANG Shu-zhen1, WANG Dong-mei1,2, LIU En-ping1,2
1. Institue of Tropical Biotechnology; Chinese Academy of Tropical Agricultural Sciences 571101, China; 2. Envirorsmental Safety Supervision and Inspection Centre for Genetically Modified Plants artd Microorganisms used in Plants, Ministry of Agriculture, HaiKou, 571101, Chin
Effect of chitinase and β-1,3- glucanase transgenic sugarcane on microbial diversity in rhizosphere soil was studied by Biolog plates method. The results showed that the Average Well Color Development(AWCD) of transgenic sugarcane was higher than non-transgenic sugarcane, indicating the carbon utilization capability of microbial community was higher. The indices of Shannon(H), Smipson(1/D) and Mcintosh(U) were calculated respectively to show the richness, dominance and evenness of the functional diversity accordingly. The results showed that the rhizosphere soil microbial diversity indices of transgenic sugarcane were all higher than those of non-transgenic sugarcane;the dominance of the functional diversity was remarkably improved, followed by the evenness;the difference of the richness was not significant. It indicated that the root exudates of transgenic sugarcane in the growing season had stimulated the growth of rhizosphere soil microbial, which might have induced the development of the microbial community with certain physical characteristics. This preliminary study showed transgenic sugarcane might affect the microbial community composition in rhizosphere soil.
谭燕华1,2*, 彭存智1,2, 张树珍1, 王冬梅1,2, 刘恩平1,2. 转基因甘蔗对根际土壤微生物多样性的影响[J]. , 2010, 29(3): 242-246.
TAN Yan-hua1,2, PENG Cun-zhi1,2, ZHANG Shu-zhen1, WANG Dong-mei1,2, LIU En-ping1,2. Effect of transgenic sugarcane on microbial community diversity in rhizosphere soil. , 2010, 29(3): 242-246.
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