解偶联蛋白2(uncouplingprotein 2,UCP2)可使氧化磷酸化解偶联。本研究首次成功从斑鳢(Channa maculata)肝脏通过简并引物克隆获得UCP2基因cDNA核心序列,该片段长502bp,编码167个氨基酸残基。使用vector NTI suite 6.0软件进行氨基酸同源性序列比较分析表明,斑鳢UCP2与真鲷、鲤鱼、草鱼、斑马鱼UCP2同源性高达91%、73%、72%、71%,与人、大鼠、小鼠UCP2同源性较高为70%、71%、70%。UCP2编码区在鱼类、哺乳类中均具有较高保守性,提示着脊椎动物UCP2可能在线粒体有氧呼吸代谢过程中承担某种最基本的生命功能。
Uncoupling protein 2 could cause oxidation phosphate uncoupling. The cDNA fragment encoding 167 amino acids of 502 bp from the liver of Taiwan snakehead(Channa maculata) were obtained by using PCR two degenerated primers. Comparison of the amino acids of Taiwan snakehead UCP2 with red sea bream(Pagrus major), Carp(Cyprinus carpio), Grass carp(Ctenopharyngodon idella), Zebrafish(Danio rerio), rat(Rattus norvegicus), mouse(Mus musculus) and human(Homo sapiens) UCP2 showed that, identity respectively is 91%、73%、72%、71%、71%、70%、70%. The high homology between the UCP2 gene of freshwater fish and mammals may explain the special function of this gene UCP2 may be a mechanism for decreasing the concentrations of reactive oxygen species(ROS) inside mitochondria.
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