Agrobacterium rhizogenes R1000 and R1601 were used to induce hairy roots from sterile cotyledons leaf and stems of Trichosanthes bracteata Voigt, the hairy root system was established by conducting inducement under various conditions. The results showed that temperature set at 250C, activating bacteria content fsxed with optical density OD600 0.7, solution plt value maintained at 6.0 and no addition of hormone level were better conditions for inducement, positive detection of agropine in the resulted hairy roots Orove transformation successful.
徐明芳1, 曹焕生2, 李定坚2. 不同培养条件对大苞栝楼发根诱导的研究[J]. , 2003, 22(3): 209-212.
XU Ming-fang1, CAO Huan-sheng2, LI Ding-jian2. The study on the effect of different conditions on inducement of hairy roots of Trichosanthes bracteata Voigt. , 2003, 22(3): 209-212.
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