Colonial cell growth of Dunaliella salina and Platymonas subcordiformis under different inoculation density and nitrogen concentration
HUANG Wei-wei1,2, CAI Zhuo-ping1,3, XIAO Qun1, DUAN Shun-shan1
1. Institute of Hydrobiology, Jinan University, Guangzhou 510632, China; 2. College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; 3. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
We investigated the colonial cell growth of marine economic microalgae,Dunaliella salina and Platymonas subcordiformis under different cell inoculation densities(10:0,7:3,5:5,3:7 and 0:10) and nitrogen concentrations(low nitrogen:1.4 mg·L-1, medium nitrogen:14 mg·L-1 and high nitrogen:140 mg·L-1) by examining some parameters of algal cell density,biomass,chlorophyll a and protein content.The results indicated that colonial cell growth of these two algae increased with the increasing of nitrogen concentrations.Besides,cell growth of mixed-culture was superior to monoculture when at high and medium nitrogen concentrations; especially when they were cultured at a proportion of 7:3.For example,chlorophyll a content was increased by as high as 1.17(p<0.01) and 7.77 folds(p<0.01),and protein content was improved by 19.1%(p<0.05) and 195.3%(p<0.01),respectively.However,cell growth of mixed-culture was remarkably limited by nitrogen concentration when at low nitrogen concentration.
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