不同盐度和Fe3+浓度对小球藻生长、硝酸还原酶活性及基因表达的影响

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摘要

论文以一株紫外诱变获得的蛋白核小球藻F-9-3为材料,研究了4个盐度和5个Fe³⁺浓度对其生长和硝酸还原酶(NR)活性及其基因表达的影响.盐度实验结果表明在0～0.30 mol·L^-1NaCl浓度范围内,小球藻生长较快;NR酶活性及其基因表达量都是在0.15和0.30 mol·L^-1NaCl较高.铁浓度实验结果表明在0.03和0.06 mmol·L^-1Fe³⁺浓度培养小球藻生长较快,NR酶活性和基因表达量较高;而高铁浓度组(0.12 mmol·L^-1)其生长受抑制,缺铁情况下nr基因表达量最高.因此适合该小球藻生长的最适盐度范围是0～0.30 mol·L^-1NaCl和0.03～0.06 mmol·L^-1Fe³⁺浓度

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	杜宇
	孙雪
	徐年军

关键词 ：小球藻, 盐度, Fe3+浓度, 硝酸还原酶

Abstract：

The effects of four different salinity and five iron concentrations on the growth,nitrate reductase(NR)activity and its expression were investigated in Chlorella pyrenoidosa F-9-3,which was selected after ultraviolet radiation.The salinity experiment showed that C.pyrenoidosa F-9-3 grew faster in 0-0.30mol·L^-1 NaCl medium than in 0.45 mol·L^-1 NaCl medium.And NR activity and its expression were higher in 0.15 and 0.30mol·L^-1 NaCl groups than those of other two groups.The iron experiment showed that the growth,NR activity and gene expression of C.pyrenoidosa F-9-3 were all higher in 0.03 and 0.06 mmol·L^-1 Fe³⁺ treatments than those in other Fe³⁺ concentration mediums.The growth was inhibited in 0.12 mmol·L^-1 iron medium and NR expression was the highest in iron deficiency group.It can be concluded that optimum culture condition of the alga was 0-0.30 mol·L^-1 NaCl and 0.03-0.06 mmol·L^-1 iron concentration.

Key words： Chlorella salt Fe3+ concentration nitrate reductase gene expression

收稿日期: 2012-08-25

基金资助:浙江省科技厅公益性研究项目(2010C33066);浙江省创新团队项目(2009R50012-6);宁波市科技攻关项目(2010C10022);宁波市国际合作项目(2010C10022)

引用本文:

杜宇, 孙雪*, 徐年军. 不同盐度和Fe3+浓度对小球藻生长、硝酸还原酶活性及基因表达的影响[J]. , 2012, 31(4): 441-445.
DU Yu, SUN Xue, XU Nian-jun. Effects of different salinity and iron concentration on growth, nitrate reductase activity and its expression in Chlorella. , 2012, 31(4): 441-445.

链接本文:

http://www.ecolsci.com/CN/Y2012/V31/I4/441

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