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盐生杜氏藻对盐度改变的生理响应
吴春, 段舜山*
暨南大学水生生物研究所, 广州, 510632
Response of Dunaliella salina to the salinity’s change
WU Chun, DUAN Shun-shan
Institute of Hydrobiology, Jinan University, Guangzhou 510632, China
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摘要 

以盐生杜氏藻为实验材料,采用f/2培养基,设置了8个盐度(15、20、25、30、50、70、90、110)处理,分盐度改变前(A)和盐度改变后(B)两个实验阶段,研究了盐生杜氏藻在不同盐度处理下的生长情况,测定了藻液的OD值、叶绿素a、β-胡萝卜素、可溶性蛋白质和可溶性糖含量等指标。结果表明,A阶段,几个较低盐度(15、20、25和30)处理生长状况较好,其中又以盐度20的处理最好;余下的处理,盐度越高,其生长所受的影响越大。B阶段,盐生杜氏藻的生长进入平台期后,50、70、90、110几个盐度较高处理的细胞密度、叶绿素a、β-胡萝卜素含量均显著超过了作为对照的盐度20的处理。且B阶段末期,先前盐度15的处理蛋白质、糖的积累量,与A阶段末期相比都有了不同程度的增加,而其余盐度处理组的蛋白质、糖含量则分别产生了不同程度的下降。

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吴春
段舜山
关键词 盐生杜氏藻盐度改变响应    
Abstract

Dunaliella salina was used as the stuff for our experiment, and f/2 culture medium were used as the culture medium. Set eight salinity treatments(15、20、25、30、50、70、90 and 110). And in two phases which the one before the salinity changed(A) and the other after the salinity changed(B), the growth status of Dunaliella salina under different salinity treatments were studied and some index were tested such as the value of OD、the content of chlorophyll-a and β-carotene、the content of dissolvable protein and carbohydrate. So the result showed that in the first stage(A), Dunaliella salina under some low salinity treatments(15、20、25 and 30) grew well, especially under the salinity treatment(20); but for the other ones, the salinity was higher, their growth were affected much more. In the second stage(B), when the growth of Dunaliella salina went to the exponential phase, the cell density, content of chlorophyll-a、β-carotene of this algae under the four salinity treatments(50、70、90、110 ) had all exceeded the one which grew under the salinity(20) treatment evidently. And at the end of the second stage(B), the content of protein and carbohydrate of Dunaliella salina under the salinity(15) treatment had increased compared with the one at the end of the first stage(A), but the content of protein and carbohydrate of Dunaliella salina grew under other salinity treatments had all decreased.

Key wordsDunaliella salina    Salinity change    Response
收稿日期: 2006-04-25     
:  Q949.2  
基金资助:国家自然科学基金(30370231);广东省科技计划重点引导项目(2005B33201001);珠海市科技计划重大项目(PB20041018)
引用本文:   
吴春, 段舜山*. 盐生杜氏藻对盐度改变的生理响应[J]. , 2006, 25(2): 135-138.
WU Chun, DUAN Shun-shan. Response of Dunaliella salina to the salinity’s change. , 2006, 25(2): 135-138.
链接本文:  
 http://www.ecolsci.com/CN/Y2006/V25/I2/135
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