Effect of glucose and sodium acetate on mixtrophic growth of Isochrysis zhanjiangensis
HE Dong-mei, WANG Na, SUN Kai-feng, HUANG Zhen-hua, DUAN Shun-shan
Department of Ecology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou, 510632, China
Effects of exogenous organic carbons including glucose and sodium acetate on the growth and total lipid content of Isochrysis zhanjiangensis were investigated in this study.The results showed that with the increasing of concentrations of glucose and sodium acetate,the growth and total lipid content increased first and then decreased.The upper limits of concentrations of glucose and sodium acetate for increasing the growth were 50 and 30g·L-1 respectively.The biomass of I.zhanjiangensis was significantly increased when the glucose was 15g·L-1,or sodium acetate was 2.5-15g·L-1.Glucose of 1.0-50g·L-1 and sodium acetate of 1.0-15g·L-1 promoted the total lipid content of I.zhanjiangensis.Glucose of 15g·L-1,or sodium acetate of 7.5g·L-1 increased the biomass and total lipid content by 30%.The results indicate that glucose and sodium acetate can obviously increase the biomass and total lipid content,and that their optimal concentrations were 1.5 and 7.5g·L-1.The ability of I.zhanjiangensis for utilizing glucose and sodium acetate is limited for mixotrophic growth.
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