土壤微生物生态学研究中的非培养方法

摘要
图/表
参考文献
相关文章 (3)

全文: PDF (405 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

土壤微生物种类和数量是评价土壤健康质量的重要指标之一。然而环境中90%以上的微生物不能够通过传统的培养基培养方法获得。最近发展起来的分析方法如磷脂脂肪酸(PLFA)、BIOLOG微孔板和分子生物学的方法可从不同方面对土壤微生物群落进行更为详尽的分析。论文就土壤微生物生态学研究中使用的主要方法进行了综述。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	韩雪梅
	郭卫华
	周娟
	张淑萍
	王仁卿

关键词 ：土壤微生物生态, 磷脂脂肪酸, BIOLOG微孔板, G+C含量, 16SrDNA

Abstract：

Soil microbiological analyses may serve as a means for assessing soil characteristics.Standard microbiological culture-techniques,however,leave over 90% of the microorganisms in the environment unaccounted for.Several more recently developed analytical techniques such as phospholipid fatty acids(PLFA),BIOLOG microplates,and molecular techniques allow for more detailed analyses of soil microbial communities.Each of these methods analyzes a different aspect of soil microbial characteristics.This review provides an outline of the main methods used in soil microbial ecology.

Key words： Soil microbial ecology PLFA Biolog microplate G+C content 16S rDNA

收稿日期: 2006-02-25

Q14

基金资助:山东省优秀中青年科学家科研奖励基金(No.2005BS08010);国家重大地质调查项目(No.1212010310306);教育部博士点基金(No.20030422030)

引用本文:

韩雪梅, 郭卫华, 周娟, 张淑萍, 王仁卿*. 土壤微生物生态学研究中的非培养方法[J]. , 2006, 25(1): 87-90.
HAN Xue-mei, ZHOU Juan, GUO Wei-hua, ZHANG Shu-ping, WANG Ren-qing. Culture-independent Approaches for Assessing Soil Biological Characteristics. , 2006, 25(1): 87-90.

链接本文:

http://www.ecolsci.com/CN/Y2006/V25/I1/87

[1] Roselló-Mora R,Amann R.2001.The species concept for prokaryotes[J].FEMS Microbiology Reviews,25(1):39-67.

[2] Erb R W,Wagner-Dobler L.1993.Detection of polychlorinated biphenyl degradation genes in polluted sediments by direct DNA extraction and polymerase chain reaction[J].Applied and Environmental Microbiology,59(12):4065-4073.

[3] Langworthy T A.1985.Lipids of Archaebacteria[J].The Bacteria,8:459-497.

[4] White D C,Davis W M,Nickels J S,et al.1979.Determination of sedimentary microbial biomass by extractable lipid phosphate[J].Oecologia,40:51-62.

[5] Ringelberg D B,Davis J D,Smith G A,et al.1989.Validation of signature polarlipid fatty acid biomarkers for alkane-utilizing bacteria in soils and subsurface aquifer materials[J].FEMS Microbiology Ecology,62:39-50.

[6] Bligh E G,Dyer W J.1959.A rapid method of total lipid extraction and purification[J].Canadian Journal of Biochemistry Physiology,37:911-917.

[7] Frostegard A,Baath E.1996.The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil[J].Biology and Fertility of Soils,22:59-65.

[8] Fritze H,Pietikainen J,Pennanen T.2000.Distribution of microbial biomass and phospholipid fatty acids in podzol profiles under coniferous forest[J].European Journal of Soil Science,51:565-573.

[9] White D C,Stair J O,Ringelberg D B.1996.Quantitative comparisons of in situ microbial biodiversity by signature biomarker analysis[J].Journal of Industrial Microbiology,17:185-196.

[10] Zelles L.1997.Phospholipid fatty acid profiles in selected members of soil microbial communities[J].Chemosphere,35:275-294.

[11] Borga P,Nilsson M,Tunlid A.1994.Bacterial communities in peat in relat ion to botanical composition as revealed by phospholipid fatty-acid analysis[J].Soil Biology and Biochemistry,26:841-848.

[12] Steinberger Y,Zelles L,Bai Q Y,et al.1999.Phospholipid fatty acid profiles as indicators for the community structure in soils along a climatic transect in the Judean Desert[J].Biology and Fertility of Soils,28:292-300.

[13] Garland J L,Mills A L.1991.Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization[J].Applied and Environmental Microbiology,57:2351-2359.

[14] Fredrickson J K,Balkwill D L,Zachara J M,et al.1991.Physiological diversity and distributions of heterotrophic bacteria in deep Cretaceous sediments of the Atlantic coastal plain[J].Applied and Environmental Microbiology,7:402-411.

[15] Guckert J B,Carr G J,Johnson T D,et al.1996.Community analysis by Biolog:curve integration for statistical analysis of activated sludge microbial habitats[J].Journal of Microbiological Methods,27:183-197.

[16] Hollibaugh J T.1994.Relationship between thymidine metabolism,bacterioplankton community metabolic capabilities,and sources of organic matter.Microbial Ecology,28:117-131.

[17] O'Connell S P,Garland,J L.2002.Dissimilar response of microbial communities in Biolog GN and GN2 plates[J].Soil Biology and Biochemistry,34:413-416.

[18] Choi K H,Dobbs F C.1999.Comparison of two kinds of Biolog microplates(GN and ECO) in their ability to distinguish among aquatic microbial communities[J].Journal of Microbiological Methods,36:203-213.

[19] Garland J L,Lehman R M.1999.Dilution/extinction of community phenotypic characters to estimate relative structural diversity in mixed communities[J].FEMS Microbiology Ecology,30:333-343.

[20] Franklin R B,Garland J L,Bolster C H,et al.2001.Impact of dilution on microbial community structure and functional potential:comparison of numerical simulations and batch culture experiments[J].Applied and Environmental Microbiology,67:702-712.

[21] Herrick J B,Madsen E L,Batt C A,et al.1993.Polymerase chain reaction amplification of naphthalene-catabolic and16S rRNA gene sequences from indigenous sediment bacteria[J].Applied and Environmental Microbiology,59(3):687-694

[22] Torsvik V L.1980.solation of bacterial DNA from soil[J].Soil Biology and Biochemistry,12:15-21.

[23] Torsivk v,Goksoyr J,Daae F L.1990.High diversity of DNA of soil bacreia[J].Applied and Environmental Microbiology,56:782-787.

[24] Torsivkv,Salte K,sorheim R,et al.1990.Comparison of phenotypic diversity and heterogeneity in a population of soil bacteria[J].Applied and Environmental Microbiology,56:776-781.

[25] Goodfellow M,O'Donnell A G.1993.Roots of bacterial systematics.In:Goodfellow,M.,O'Donnell,A.G.(Eds.),Handbook of New Bacterial Systematics.Academic Press Inc.,San Diego.pp.3-56.

[26] Vandamme P,Pot B,Gillis M,et al.1996.Polyphasic taxonomy,a concensus approach to bacterial systematics[J].Microbiological Review,60:407-438.

[27] Santo Domingo J W,Kaufman M G,Klug M J,et al.1998.Influence of diet on the structure and function of the bacterial hindgut community of crickets[J].Molecular Ecology,7:761-767.

[28] Woese C R.1987.Bacterial evolution[J].Microbiological Review,51:21-271.

[29] Olsen G J,Woese C R,Overbeek R.1994.The winds of(evolutionary) change:breathing new life into microbiology[J].Journal of Bacteriology,176:1-6.

[30] Muyzer G A,de Waal E C,Uitterlinden A G.1993.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reactionamplified genes coding for 16S rRNA[J].Applied and Environmental Microbiology,59:695-700.

[31] Lee D H,Zo Y G,Kim S J.1996.Nonradioactive method to study genetic profiles of natural bacterial communities by PCR-single-strand-conformation polymorphism[J].Applied and Environmental Microbiology,62:3112-3120.

[32] Smit E,Leeflang P,Wernars K.1997.Detection of shifts in microbial community structure and diversity in soil caused by copper contamination using amplified ribosomal DNA restriction analysis[J].FEMS Microbiology Ecology,23:249-261.

[33] Widmer F,Seidler R J,Watrud L S.1996.Sensitive detection of transgenic plant marker gene persistence in soil microcosms[J].Molecular Ecology,5:603-613.