Effects of 5 kinds of antibiotics (chloramphenicol, ampicillin, streptomycin, kanamycin, gentamycin) at different concentrations on the growth of Isochrysis zhangjiangensis were studied, the axenic culture was obtained by mixed antibiotics. The results showed that:(1) There were differences among different antibiotics, chloramphenicol was the most susceptible antibiotic, the inhibition rate was 27.02% at the concentration of 50 μg·mL-1, more than 50 μg·mL-1 the inhibition rate was 80%. I.zhangjiangensis was not susceptible to ampicillin, streptomycin, kanamycin and gentamycin, there was stimulation at the lower concentration of the last three antibiotics. (2) Except for ampicillin at the concentration of 50 μg·mL-1, there were bacteriostasis effects of the other treatments. (3) Mixed antibiotics (500 μg·mL-1streptomycin,1000 μg·mL-1 kanamycin, and 50 μg·mL-1 gentamycin) were added to the culture medium by 4 combinations, the axenic culture was obtained, and there were stimulation on the growth of I.zhangjiangensis.
黄振华, 刘晓娟, 胡章喜, 段舜山*. 湛江等鞭金藻对抗生素的反应及无菌化培养[J]. , 2007, 26(2): 120-121.
HUANG Zhen-hua, LIU Xiao-juan, HU Zhang-xi, DUAN Shun-shan. Effects of antibiotics on the growth of Isochrysis zhangjiangensis and axenic culture. , 2007, 26(2): 120-121.
