We evaluated the performance of an imported microcystin ELISA(enzyme-linked-immuno-absborbed assay) kit in the study.The ELISA kit was evaluated by the precision test, MC-LR standard recovery test, cross-reactivity test and a test of comparison with an ELISA method based on the monoclonal antibodies(M8H5).The CVs for intra-assay was very low and that for inter-assay was relatively high.Recovery rate was from 73.5%to 97.8%.The kit showed a high cross-reactivity rate to MC-LR, but a low cross-reactivity rate to other microcystin variants such as MC-RR, MC-LW and MC-LF.ELISA analysis of water samples revealed that the results from two ELISA methods were nearly consistent.The Beacon microcystin plate kit can meet the requirements for qualitative and quantitative detection of MC-LR in water.
[1] de Figueiredo D R, Azeiteiro U M, Esteves S, et al.2004.Microcystin producing blooms-a serious global public health issue[J].Ecotoxicology and envitonmental safety, 59:151-163.
[2] Yu S Z.1995.Peimary prevention of hepatocellular carcinoma[J].Gastroenterol.Hepatol, 10:674-682.
[3] Rapala J., Erkomaa K., Kukkonen J., et al.2002.Detection of microcystins with protein phosphatase inhibition assay, high performance liquid chromatography-UV detection and enzymelinked immunosorbent assay[J].Comparison of methods Anal Chim Acta., 466:213-231.
[4] 雷腊梅, 甘南琴, 宋立荣, 等.2004.三种检测微囊藻毒素的ELISA方法比较研究[J].高技术通讯, 7:89-92.
[5] 孔繁翔, 高光.2005.大型浅水富营养化湖泊中蓝藻水华形成机理的思考[J].生态学报, 25(3):589-595.
[6] Chu F S, Huang X, Wei R D, et al.1989.Production and characterization of antibodies against microcystins[J].Appl Environ Microbiol, 55:1928-1933.
[7] Metcalf J S, Bell S G, Codd G A.2000.Production of nuvel polyclonal antibodies against the cyanobacterail toxin microcystin-LR and their application for the detection and quantification of microcystins and nodularin
[1] .Wat Res, 34:2761-2769.
[8] Zeck A, Eikenberg A, Weller M G, et al.2001.Highly sensitive immunoassay based on amonoclonal antibody specific for[4-arginine]microcystins[J].Ana.Chim Acta, 441:1-13.
[9] Yu F Y, Liu B H, Chou H N, et al.2002.Development of a sensitive ELISA for the determination of microcystins in algae[J].Agric Food Chem, 50:4176-4182.
[10] Nagata S, Soutome H, Tsutsumi T.et al.1995.Novel monoclonal antibodies against microcystin and their protective activity for hepatotoxicity[J].Nat Toxins, 3(2):78-86.
[11] Oudra B, Loudiki M, Vasconcelos V, et al.2002.Detection and quantification of microcystins from cyanobacteria strains isolated from reservoirs and ponds in Morocco[J].Environ Toxicol., 17(1):32-39.
[12] Sotero-Santos R B, Carvalho E G, Dellamano-Oliveira M J.et al.2008.Occurrence and toxicity of an Anabaena bloom in a tropical reservoir(Southeast Brazil)[J].Harmful Algae, 7:590-598.
[13] 李金明.2005.临床酶免疫测定技术[M].北京:人民军医出版社, 87-92.
[14] Hyenstrand P, Metcalf J S, Beattie K A, et al.2001.Losses of the cyanobacterial toxin microcystin-LR from aqueous solution by adsorption during laboratory manipulations[J].Toxicon, 39:589-594.
[15] Falconer I R, Humpage A R.2005.Health risk assessment of cyanobacterial(blue-green algal)toxins in drinking water[J].Int J Environ Res Public Health., 2(1):43-50.
[16] Mikhailov A, H(a)rm(a)l(a) A-S, Meriluoto J, et al.2001.Production and specicity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine[J].Toxicon, 39:477-483.
[17] Sheng J W, He M, Shi H C, et al.2006.A comprehensive immunoassay for the detection of microcystins in waters based on polyclonal antibodies[J].Analytica Chimica Acta, 572:309-315.
[18] Campàs M, Marty J L.2007.Highly sensitive amperometric immunosensors for microcystin detection in algae[J].Biosensors and Bioelectronics, 22:1034-1040.