Microsystin-detoxifizyme gene plays an important role in microcystin detoxication in freshwater fishes. cDNA fragments that encoding partial sequences of microsystin-detoxifizyme gene were successfully amplified by RT-PCR using degenerate primers from the liver of the main Chinese freshwater fishes, including silver carp, bighead carp, grass carp, goldfish, Chinese perch and Tilapia. Homologous analysis show that the deduced amino acid sequences of the microsystin-detoxifizyme gene in silver carp, bighead carp, grass carp, goldfish, Chinese perch and Tilapia were 50%-60% homologus to the glutathione S-transferase gene of mammals, such as human, mouse, rat, cow, pig and sheep. The homologous analysis results indicate a big difference between the microcystin-detoxifizyme gene of freshwater fishes and the glutathione S-transferase gene of mammals, which may explain the special function of this gene in freshwater fishes for microcystin detoxication.
廖婉琴, 梁旭方*, 王琳, 方玲, 雷腊梅, 韩博平. 淡水鱼类微囊藻毒素去毒酶基因的克隆[J]. , 2005, 24(1): 6-8.
LIAO Wan-Qin, LIANG Xu-Fang, WANG Lin, FANG Ling, LEI La-Mei, HAN Bo-Ping. Cloning of the microcystin-detoxifizyme gene in the main Chinese freshwater fishes. , 2005, 24(1): 6-8.
[1] Krienitz L, Ballot A, Kotut K, et al. 2003. Contribution of hot spring cyanobacteria to the misterious deaths of lesser Flamingos at Lake Bogoria, Kenya[J]. FEMS Microbiol. Ecol.43, 141-148.
[2] 汤钊猷,余业勤.1989.原发性肝癌[M].上海:上海科学技术出版社,30-37.
[3] Sivonen K, Niemela S I, Lepisto L, et al. 1990. Toxic cyanobacterial (blue-green algae) in finish fresh and canstal waters[J] Hydrobiologia, 190: 267-2751.
[4] Chorus I, Falconer I R, Salas H J, et al., 2000. Health risks caused by freshwater cyanobacteria in recreational waters[J].J. Toxicol. Environ. Health 3, 323-347.
[5] Hitzfeld B C, Hoger S J, Dietrich R. 2000. Cyanobacterial toxins: removal during drinking water treatment, and human risk assessment[J]. Environ. Health Perspect. 10, 113-122.
[6] 谢平.2003.鲢、鳙与藻类水华控制[M].北京:科学出版社,1-134.
[7] Pfugmacher S, Wiegand C, Oberemm A, et al. 1998.Identification of an enzymatically formed glutathione conjugate of the cyanobacterial hepatotoxin microcystin-LR: the first step of detoxication[J]. Biochim. et Biophys. Acta 1425: 527-533.
[8] Wiegand C, Pfugmacher S, Giese M H. et al. 2000. Uptake,Toxicity, and Effects on Detoxication Enzymes of Atrazine and Trifluoroacetate in Embryos of Zebrafish[J]. Ecotoxicol.and Environm. Safety 45:122-131.
[9] Best J H, Eddy F B, Codd G A. 2003. Effects of Microcystis cells, cell extracts and lipopolysaccharide on drinking and liver function in rainbow trout Oncorhynchus mykiss Walbaum. Aquat[J]. Toxicol. 64: 419-426.
