斑鳢肝脏解偶联蛋白2cDNA核心片断的克隆及分析

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Abstract

Uncoupling protein 2 could cause oxidation phosphate uncoupling. The cDNA fragment encoding 167 amino acids of 502 bp from the liver of Taiwan snakehead(Channa maculata) were obtained by using PCR two degenerated primers. Comparison of the amino acids of Taiwan snakehead UCP2 with red sea bream(Pagrus major), Carp(Cyprinus carpio), Grass carp(Ctenopharyngodon idella), Zebrafish(Danio rerio), rat(Rattus norvegicus), mouse(Mus musculus) and human(Homo sapiens) UCP2 showed that, identity respectively is 91%、73%、72%、71%、71%、70%、70%. The high homology between the UCP2 gene of freshwater fish and mammals may explain the special function of this gene UCP2 may be a mechanism for decreasing the concentrations of reactive oxygen species(ROS) inside mitochondria.

Key words： Uncoupling protein 2 Molecular clone Taiwan snakehead

Received: 25 April 2006

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	Articles by authors
	CUI Yu-min
	LI Gui-sheng
	LIAO Wan-qin
	LIANG Xu-fang

Cite this article:

CUI Yu-min,LI Gui-sheng,LIAO Wan-qin, et al. Cloning and analysis of the partial cDNA sequence of Taiwan snakehead(Channa maculata) uncoupling protein 2 Gene[J]. , 2006, 25(2): 151-154.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2006/V25/I2/151

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