微囊藻毒素降解酶基因cDNA部分序列的克隆及检测分析

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Abstract

Microcystinase plays an important role in the biodegradation of microcystin in physical environment. Partial cDNA sequence of microcystinase (MlrA) gene from water body was obtained by PCR, which was 342 bp in length and encoded 113 amino acids (GenBank ID: FJ972202). MlrA was detected in water and fish body in different seasons by PCR methods. Homology of the MlrA amino acid sequence was 96% and 89% with the MlrA from Sphingomonas sp. MD-1 and Y2 published by saito in Japanese, and also was 95% and 94% with the MlrA from Sphingomonas sp.ACM-3962 in Norway and Sphingopyxis sp. LH21 in Australia, confirming that the sequences was cloned from Sphingomonas sp.. At the same time, we detected part of the fish and water in some seasons, and found the detection of MlrA gene showed positive in April and May, but that of the water detected in the other 6 months, and the fish detected in June and October showed negative.

Key words： Microcystin microcystinase gene cloning detection of gene in water and fish body

Received: 25 April 2013

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	Articles by authors
	LIANG Xu-fang
	BAI Xiao-li
	CHENG Wei-xuan
	HE Shan
	SHEN Dan

Cite this article:

LIANG Xu-fang,BAI Xiao-li,CHENG Wei-xuan, et al. Molecular cloning and detection of partial cDNA sequence of microcystinase gene[J]. , 2013, 32(2): 183-188.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2013/V32/I2/183

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