铜绿微囊藻藻毒素提取液对小鼠淋巴细胞DNA损伤的研究

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Abstract

We studied the toxic effect of algal toxin MCLR crude extraction,preliminary purified extraction and purified MCLR produced by laboratory-cultured Microcystis aeruginosa on mouse lymphocytes by using single cell gel electrophoresis(SCGE).The results showed that large amount of pigments and lipids in the crude extraction were removed by the n-hexane extraction in the preliminary process.When MCLR concentrations in crude extraction were higher than 66.2μg·L^-1,DNA damages could be induced;whereas when MCLR concentrations in preliminary purified extraction were higher than 125μg·L^-1,DNA damages were induced.The tail DNA% and OTM of preliminary purified extraction were slightly less than those of crude extraction.However,the damages of purified MCLR were much less than those of crude extraction.The damages for purified MCLR with the concentration of 494μg·L^-1 were similar to those of crude extraction with 75.9μg·L^-1 MCLR.Major parts of organic substances with UV adsorption,which were removed in the MCLR purification process,might bring about the significant differences between the crude extraction and purified MCLR.

Key words： algal toxin MCLR single cell gel electrophoresis genotoxicity

Received: 25 April 2011

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	Articles by authors
	SUI Li-li
	LIANG Wen-yan
	CHEN Li
	RUAN Ling-ling
	XU Jia
	LIANG Zhi-xia

Cite this article:

SUI Li-li,LIANG Wen-yan,CHEN Li, et al. Study on DNA damages of mouse lymphocytes induced by algal toxin extraction of Microcystis aeruginosa[J]. , 2011, 30(2): 122-127.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2011/V30/I2/122

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