低温驯化和外源糖对爪哇伪枝藻冷胁迫的影响

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Abstract

Desert algae have a broad distribution in terrestrial environments,and the algae have been proved to be able to well adapt to extreme environments.Low temperature or cold stress is one of important ecological factors influencing survival of desert algae.In this study,a typical cyanobacteria species,Scytonema javanicum(Kütz.)Born et Flah,was isolated from desert algal crusts and selected as research material.In the experiments,2℃,10℃ and 28℃(as control)were selected for temperature treatments,and simultaneously low temperature domestication and exterior sugars such as sucrose and glucose were used to treat the algal cultures under different low temperatures.The results showed that low temperatures resulted in an obvious decrease in biomass and PS Ⅱ activity in S.javanicum. Low temperature domestication and exterior sugars promoted chlorophyll a content and PS Ⅱ activity of the alga to some extent,and low temperature domestication obviously increased intracellular production of water-soluble proteins;while no influence on MDA content was observed.In addition,we found that low temperature domestication evidently decreased secretion of EPS,but exterior sugars had no obvious role in relative osmotic activities of S.javanicum.The results suggest that low temperature domestication and exterior sugars may be crucial for tolerance of S.javanicum to low temperature or cold stress.

Key words： desert alga S.javanicum low temperature stress low temperature domestication exterior saccharides endurance of cold stress

Received: 25 April 2011

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	Articles by authors
	RAO Ben-qiang
	WU Pei-pei
	LI Dun-hai
	CHEN Kun
	LIU Yong-ding

Cite this article:

RAO Ben-qiang,WU Pei-pei,LI Dun-hai, et al. Influences of low temperature domestication and exterior saccharides on endurance of Scytonema javanicum to cold stress[J]. , 2011, 30(2): 128-134.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2011/V30/I2/128

[1] 杨晓辉,张克斌,赵石杰.生物土壤结皮——荒漠化地区土壤研究的热点问题[J],生态学报,2001.21(3):474-480.

[2] Belnap,J.The world at your feet:desert biological soil crusts[J].Frontiers in Ecology ＆ the Environment,2003,1:181-189.

[3] 陈兰洲,刘永定,李敦海,沈银武,谢作明.荒漠藻类及其结皮的研究[J].中国科学基金,2003,17(2):90-93.

[4] Painter T J.Carbohydrate polymets in desert reclamation:the potential of microalgal biofertilizers[J].Carbohydrate Polymers,1993,20:77～86.

[5] 陈兰洲,谢作明,李敦海,刘永定,王高鸿.失水-吸水过程中微鞘藻光合活性的特性[J].水生生物学报,2006,30(4):404-407.

[6] Yosluyuki Tamaru,Yayoi Takani,Takayula Yoshida,Toshio sakamoto.Crucial Role of Extracellular Polysaccharides in Desiccation and Freezing Tolerance in the Terrestrial Cyanobacterium Nostoc commune[J].Applied and Environmental Microbiology,2005,71:7327-7333.

[7] D.Tang,S.Shi,D.Li,C.Hu,Y.Liu.Physiological and biochemical responses of Scytonema javanicum (cyanobacterium) to salt stress[J].Journal of Arid Environments,2007,71:312-320.

[8] 王伟波,饶本强,沈银武,李敦海,刘永定,几种固沙荒漠藻的温度适应性[J].水生生物学报,2010,34(3):489-494.

[9] 陈兰洲,刘永定,李敦海.盐胁迫对爪哇伪枝藻(Scytonema javanicum)生理生化特性的影响[J].中国沙漠,2003,23(3):285-288.

[10] WintermansJ F G M,De Mots A.Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol[J].Biochimica et Biophysica Acta,1965,109:448-453.

[11] 李合生,孙群,赵世杰,章文华.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.

[12] 张维杰,复合多糖生化研究技术[M].上海:上海科学技术出版,1987.

[13] 夏丽,陈贻竹,盐藻的低温光抑制[J].海洋科学,2002,5:56-59.

[14] Huner N P A,Oquist G,SarhanF.Energy balance and acclimation to light and cold[J].Trends to Plants Sciences,1998,3:224-230.

[15] Hillwell B,Gutteridge J M C.Free Radicals in Biology and Medicine[M].3rd Edition,Oxford:Clarendon Press,1999.

[16] 宫相忠,唐学玺.等鞭金藻8701的耐低温机理[J].水产学报,2005,5(1):20-25.

[17] 丁元丰.植物耐低温的分子机理[J].北京农业职业学院学报,2006,20(1):16-19.

[18] Bohnert H J,Nelson D E,Jensen R G.Adaptations to environmental stresses[J].Plant Cell,1995,7:1099-1111.

[19] Lee C W B,DasGuptaS K,Mattai J,Shipley G G Abdel-Mageed O H,Makriyannis A,Griffin R G.Characterization of the L-phase in trehalose-stabilization dry membranes by solid-state NMR and X-ray diffraction[J].Biochemistry,1989,25:5000-5009.

[20] Page-Sharp M D,Behm C A,Snuth G D.Involvement of the compatible solutes trehalose and sucrose in the response to salt stress of a cyanobacterial Scytonema species isolated from desert soils[J].Biochimica et Biophysica Acta,1999,1472:519-528.

[21] Oliver A E,Crowe,L M,Crowe J H.1998.Methods for dehydration-tolerance:depression of depression of the phase transition temperature in dry membranes and carbohydrate vitrification[J].Seed Science.Researchs,8:211-221.

[22] Potts M.Minireview:Mechanisms of desiccation tolerance in cyamobacteria[J].European Journal of Phycology,1999,34:319-328.

[23] Hu Chunxiang,Liu Yongding,Berit Smestad Paulsen,Dirk Petersen,Dag Klaveness.Extracellular carbohydrate polymers from five desert soil algae with different cohesion in the stabilization of fine sand grain[J].Carbohydrate Polymers,2003,54:33-42.

[24] Philippis R D,Vincenzini M.Exocellular polysaccharides from cyanobacteria and their possible applications[J].FEMS Microbiol.Reviews,1998,22:151-175.