生长阶段及环境因子对东海原甲藻氨基酸氧化酶活性的影响

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Abstract

Effects of growth phase, temperature, irradiance and nitrogen source on the amino acid oxidase (AO) activity of Prorocentrum donghaiense, a causative organism of large-scale harmful algal blooms (HABs) along the coastal waters of East China Sea, were investigated in the laboratory. The results showed that P. donghaiense displayed higher amino acid oxidase activity in N-depleted conditions. AO activity of P. donghaiense was detected at the temperatures ranging from 15 to 30℃, in which AO activity was significantly higher at lower temperatures (15℃-20℃) than that at higher temperatures(25℃-30℃) (p<0.05), and the maximum value was observed at 20℃. Although AO activity was relatively higher (0.38 to 0.47 fmol/(h·cell)) at higher irradiances (50-100 μmol/(m²·s)), AO activity was still up to 0.28 fmol/(h·cell) at irradiance of 2 μmol/(m²·s¹). Nitrogen source exhibited a significant impact on AO activity of P. donghaiense. The highest AO activity (0.44 fmol/(h·cell)) was observed when alanine was used as the sole nitrogen source, and the lowest AO activity (0.22 fmol/(h·cell)) was recorded in the combination of ammonium and alanine. It suggested that temperature, irradiance and nitrogen source were key environmental factors regulating AO activity of P. donghaiense. P. donghaiense is not only capable of utilizing free amino acids effectively, but also flexible in adapting to broad temperature and lower irradiance, which may play important roles in the formation and duration of P. donghaiense blooms.

Key words： Prorocentrum donghaiense dissolved free amino acid (DFAA) dissolved organic nitrogen (DON) amino acid oxidase (AO) environmental factor

Received: 25 February 2013

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	Articles by authors
	LIU Jing-ya
	LAI Hai-yan
	XU Ning
	DUAN Shun-shan

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LIU Jing-ya,LAI Hai-yan,XU Ning, et al. Effects of growth phase and environmental factors on amino acid oxidase activity of Prorocentrum donghaiense[J]. , 2013, 32(1): 27-33.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2013/V32/I1/27

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