4株海洋酵母菌胞外酶活力及其对水产养殖有机污染物的降解

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Abstract

Production of amylase, protease, lipase, and cellulose of four marine yeasts was compared using differential medium plate method, and the enzyme activities were also analyzed in this research. The results showed that Candida colliculosa DY11-1 could secrete amylase, protease and lipase, and their activities were 181.4, 123.1 U/mL and 54.2 U/mL, respectively. Pichia sp DY11-6 and Candida parapsilosis DY07-1 could secrete amylase and protease. Their amylase activities were 108.1 and 96.5 U/mL, and their protease activities were 35.1 and 128.4 U/mL, respectively. Rhodotorula glutinis DY02-3 did not secrete the four extracellular enzymes. Feed extracts of Tilapia mossambica were used as aquaculture organic pollutants to compare the degradation ability of the four marine yeasts. The results showed that Candida colliculosa DY11-1 possessed the strongest degradation capacity. The concentration of COD, TN and TP in the tested water decreased from 27.60, 12.22 and 2.67 mg/mL to 15.73, 7.59 and 1.40 mg/mL after 9 days, respectively, with the degradation rates of 43.0%, 37.8% and 47.5%. It indicated that DY-11 had potential application value in improving water quality of aquaculture.

Key words： marine yeast extracellular enzyme aquaculture organic pollutants depuration

Received: 25 February 2013

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	DAI Shun-zhen
	XIA Xin-jian
	XU Zhong-neng
	LIN Xiao-tao

Cite this article:

DAI Shun-zhen,XIA Xin-jian,XU Zhong-neng, et al. Studies on activities of extracellular enzymes of four yeasts and their degradation of organic pollutants from aquaculture[J]. , 2013, 32(1): 22-26.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2013/V32/I1/22

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