穿心莲内酯衍生物抗H2O2诱导胰岛RIN-mβ细胞凋亡的蛋白组学研究

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Abstract

To screen the differential proteins invovled in andrographolide analogue (AL-1) against apoptosis induced by hedrogen peroxide (H₂O₂) in Rin-m beta cells and investigate its molecular mechanism with proteomics. The results showed that:The survival rate of cells treated by H₂O₂ were increased by AL-1 in a dose-dependent manner. With proteomics analysis, 18 proteins related to apoptosis, stress were successfully identified, such as Prohibitin, Shmt2, Rho GDP-dissociation inhibitor-1, Galectin-1, Cyt b5, Hsps, and so on. Among them, 9 proteins were found to be up-regulated and 9 down-regulated in the AL-1 treated group compared with control (H₂O₂) group. AL-1 protected Rin-m beta cells against apoptosis induced by H₂O₂ via regulating proteins associated with apoptosis, stress.

Key words： andrographolide analogue (AL-1) H2O2 RIN-m beta cells two-dimensional gel electrophoresis(2-DE) proteomics

Received: 25 February 2010

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	Articles by authors
	ZHOU Hui-hua
	DING Wen
	JIA Hong-ling
	WANG Yu-qiang
	YAN Guang-rong

Cite this article:

ZHOU Hui-hua,DING Wen,JIA Hong-ling, et al. Proteomic study of andrographolide analogue mediated protection on cell apoptosis induced by H2O2 in RIN-m beta cells[J]. , 2010, 29(1): 80-84.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2010/V29/I1/80

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