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Genetic variation of lacustrine Plecoglossus altivelis population in Dongzhang Reservoir, Fujiang Province |
LU Yan-fu, YUE Xiao-liang, ZHAO Shuang, CHEN Quan-mei, ZHANG Qun |
Research Center of Hydrobiology, Jinan University, Guangzhou 510632, China |
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Abstract Five hundred and thirty three bp sequence of partial mitochondrial ND4-tRNASer gene of 18 individuals of lacustrine Plecoglossus altivelis population from Dongzhang Reservoir,Fujiang Province was determined,and 4 haplotypes,2 variable sites and 2 parsimony-informative sites were detected.Genetic distance based on Kimura 2-parameter model among 4 haplotypes ranged from 0.001 9 to 0.003 9.No obvious genealogical clusters were found in neighbor-joining tree.Haplotype diversity(Hd) and nucleotide diversity(Pi) were 0.627 and 0.001 37 respectively with average nucleotide difference 0.732 in the population,which were much lower than in Plecoglossus altivelis altivelis in Japan(Hd=0.793,Pi=0.002 94),but similar as compared with Plecoglossus altivelis ryukyuensis,an endangered subspecies listed in IUCN(Hd=0.519,Pi=0.001 11).Further studies are still needed to make clear the reasons responsible for such poor genetic diversities in Dongzhang reservoir populations;meanwhile urgent measures should be taken to conserve the valuable fishes.
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Received: 25 December 2009
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