基于线粒体细胞色素b基因全序列的海南长臀鮠南渡江种群遗传变异分析

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Abstract

The 1138 bp sequence of complete mitochondrial cytochrome b gene (cytb) of 15 individuals of Cranoglanis bouderius multiradiatus population in Nandujiang River, HaiNan Province were determined. 3 variable sites and 1 parsimony-informative sites were detected in 4 hapoltypes;there were a typical initiation codon (ATG) and incomplete termination codon (T^**) in the Cytb gene of Cranoglanis bouderius multiradiatus, and the content of Gs in the third codon position was only accounted for 2.9%, but the content of Cs and As was 39.1 % and 38.5% respectively. The content of Ts in the second codon position was 41.3%, indicating that the usage of 4 bases in different codon positions of cytb gene were biased. Genetic distances based on Kimura 2-parameter model among 4 haplotypes ranged from 0.0009 to 0.0017, and no obvious genealogical cluster were found in the neighbor-Joining tree. The result that haplotype diversity (Hd) and nucleotide diversity(Pi) were 0.600 and 0.0006 respectively in the population suggested that genetic diversity of sequences of cytb gene of Cranoglanis bouderius multiradiatus in the Nandujiang River was very poor, and urgent and effective measures should be taken to conserve the valuable fish.

Key words： Cranoglanis bouderius multiradiatus cytochromeb Genentic variation

Received: 25 June 2010

Q178.532

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	YUE Xiao-Hang
	ZHAO Shuang
	LIU Hai-lin

Cite this article:

YUE Xiao-Hang,ZHAO Shuang,LIU Hai-lin. Genetic variation of cranoglanis bouderius multiradiatus population in Nandujiang River base on mitochondrial cytochrome b sequences[J]. , 2010, 29(3): 247-250.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2010/V29/I3/247

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