Microscopic observation on the development of Enteromorpha prolifera (Chlorophyta)
WANG Jian-wei1, LIN A-peng1, Li Yan-yan1, SHEN Song-dong1, YAN Bin-lun2
1. Life Sciences College, Soochow University, Suzhou, 2151232, Jiangsu, China; 2. Public Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, Jiangsu, China
After the observation on the development of Enteromorpha prolifera(Müller) J.Ag.by microscope and the paraffin-section method,the microscopic characteristics of the develop process from spores to mature thalli have been expatiated.Spores of Enteromorpha prolifera(Müller) J.Ag.were developed from mature thallus cells,every mature thallus cell could release 10～30 spores.Every spore divided into 2-cell structure after the first division,and this structure had obvious polarity.The base-cell developed into rhizoid,and the top-cell formed protonema by horizontal-division continually.Pipelike thallus was formed by vertical-division of the middle protonema cells,and top-cell kept horizontal-division.Vertical-division included tangential-division and radial-division,tangential-division made the pipelike thallus more thick,and radial-division made the branch formed.The internal cavum of pipelike thallus contained villiform protuberances and meshwork structures which were composed of glycoprotein.When the number of cells in cross section of pipelike thallus reached 30～50,the villiform protuberances disappeared and the meshwork structures became tight,then the mural cells of pipelike thallus adhered and the foliolose thallus formed.The total developmental process of Enteromorpha prolifera(Müller) J.Ag.kept polarity.
WANG Jian-wei,LIN A-peng,Li Yan-yan, et al. Microscopic observation on the development of Enteromorpha prolifera (Chlorophyta)[J]. , 2006, 25(5): 400-404.
[4] Noda H,Amano H,Arashima K,et al..1990.Antitumor activity of marine algae[J].Hydrobiologia,204/205:577-584.
[5] Hiqashi-Okaj K,Otani S,Okai Y.1999.Potent suppressive effect of a Japanese edible seaweed,Enteromorpha prolifera (Sujiao-nori) on initiation and promotion phases of chemically induced mouse skin tumorigenesis[J].Cancer Lett,140:21-25.
[6] 郑国锠.1978.生物显微技术[M].北京:人民教育出版社.228.
[7] Christie A O,Evans L V.1962.Periodicity in the liberation of gametes and zoospores of Enteromorpha intestinalis Link[J].Nature,193:193-194.
[8] Dan A,Hiraoka M,Ohno M,Critchley A.2002.Observations on the effect of salinity and photon fluence rate on the induction of sporulation and rhizoid formation in the green alga Enteromorpha prolifera (Müller) J.Agardh (Chlorophyta,Ulvales)[J].Fish.Sci.,68(6):1182.
[9] Pringle J D.1986.Swarmer release and distribution of life-cycle phases of Enteromorpha intestinalis (L.)(Chlorophyta) in relation to environmental factors[J].Exp.Mar.Biol.Ecol,100:97-111.
[10] Eaton J W,Brown J G,Round F E.1966.Some observations on polarity and regeneration in Enteromorpha[J].Br.Phycol.Bull,3:53-62.
[11] Lersten N R,Voth P D.1960.Experimental control of zooid discharge and rhizoid formation in the green alga Enteromorpha[J].Bot.Gaz,122:33-45.
[12] Moss B,Marsland A.1976.Regeneration of Enteromorpha[J].Br.J.Phycol.,11:309-313.
[13] Lee T F,Wichroski M.1996.Polar regeneration in Enteromorpha prolifera[J].Phycol,32:2.
[14] Burrows E M.1959.Growth,form and environment in Entermomorpha[J].Linn.Soc.Bot.,56:204-206.
