中国沿海浒苔属rbcL和ITS序列比较及系统进化分析

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Abstract

In the present study,genetic relationship and geographic distribution of Enteromorpha genus in China coast were clarified. The samples were collected from Zhanqiao of Qingdao,Qianggang of Yancheng,Xiangshan of Ningbo and Pingyang of Wenzhou.The fragments of ITS1,5.8S rDNA and ITS2 were amplified,cloned and sequenced.The length of genetic fragments of rbcL from these four regions were all 1201bp.The nucleotide diversity and genetic distance were analyzed,and phylogenetic trees were constructed with neighbor-joining method.The results showed that the rate of evolution of ITS was the fastest,while rbcL was quite conservative.The region of ITS was short,with the content of GC up to over 65%,however the content of GC in 5.8S rDNA was 50%.ITS1 was more diverse than ITS2.There were geographic variations among the samples from the four sites.Genetic relationship between samples from Qingdao and Yancheng was closer,and so was that between samples from Xiangshan and Wenzhou.Ulva and Enteromorpha did not separate into an independent branch respectively,but they clustered together.So it can be concluded that genetic relationship between Ulva and Enteromorpha is close.Either Enteromorpha linza or Enteromorpha prolifera from Yancheng may caused the green tide in Qingdao.

Key words： Enteromorpha genus rbcL ITS phylogeny

Received: 25 December 2010

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	Articles by authors
	WANG Ya-jun
	YI Qian-qian
	WANG Gang
	SUN Xue
	YANG Rui

Cite this article:

WANG Ya-jun,YI Qian-qian,WANG Gang, et al. Phylogenetic analysis of rbcL and ITS of Enteromorpha genus from Chinese coast[J]. , 2010, 29(6): 507-511.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2010/V29/I6/507

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