The extraction of chlorophyll a from phytoplankton was by freezing-thawing was assessed in this study. Filters containing alga cells were frozen and thawed repeatedly, then were steeped in 90% acetone solution for ~20 hours. Compared to the standard rubbing method, the freezing-thawing method had the improvements on small artificial error, high consistency, high accuracy, and operational simplicity. The filteration volume had no significant effect on the results for the mono-species algal cultures (P>0.05), but Bur the results for the lake water were significantly affected by filteration volume (P<0.01). For the water samples from eutrophic waterbodies, the 100ml water volume was suggested to be filtered.The data analysis showed there was no significant difference between Acetate Fiber Filter, Tiny Aperture Filter and Glass Fiber Filter.
林少君, 贺立静, 黄沛生, 韩博平*. 浮游植物中叶绿素a提取方法的比较与改进[J]. , 2005, 24(1): 9-11.
LIN Shao-Jun, HE Li-Jing, HUNAG Pei-Sheng, HAN Bo-Ping. Comparison and improvement on the extraction method for chlorophyll a in phytoplankton. , 2005, 24(1): 9-11.
[11] Lorenzen C J. 1967. Determination of chlorophyll and pheo-pigments: spectrophotometric equations[J]. Limnology.Oceanography. 12: 343-346.
[12] Yentsch C S, Menzel D W. 1963. A method for the determination of phytoplankton chlorophyll and phaeophytin by fluorescene[J]. Deep Sea Research, 10:221-231.
[13] Schmid H, Bauer F and Stich H.1998.Determination of algal biomass with HPLC pigment analysis from lakes of different trophic state in comparison to microscopically measured biomass[J]. Journal of Plankton Research, 20: 1651-1661.
