Microorganism QK-1 was immobilized on wood crumbs and the effect of crude oil removal from aqueous solution by the immobilized QK-1 was tested. The removal rate of the crude oil by the immobilized QK-1 was higher than that by only QK-1 or the mixture of QK-1 and wood crumbs. The removal rate was 75.5~94.3% when the crude oil concentration was 10.0~30.0 g·L-1, and the optimum strain dosage was 15.0 g·L-1(dry weight). The results indicated that the culture medium was a crucial factor in oil removal, especially carbon and nitrogen contents. The optimum composition of immobilized culture medium was as follows:sucrose, 10.0 g·L-1; barmene, 6.0 g·L-1; yeast extract, 1.5 g·L-1; pH 8.0.
[6] Toshiaki Hanaoka, Hiroki Hayashi, Teruoki Tago. 2001. In situ immobilization of ultrafine particles synthesized in a water/oil microemulsion[J]. Journal of colloid and interface science, 235(2):235～240.
[7] Kowalska M, Bodzek M, Bohdziewicz J. 1998. Biodegradation of phenols and cyanides using membranes with immobilized microorganisms[J]. Process biochemistry, 33(2):189～197.
[8] Sekoulov I, Brinke-Seiferth S. 1999. Application of biofiltration in the crude oil processing industry[J]. Vater science and technology, 39(8):71～76.
