Research of ammonia nitrogen wastewater treatment using novel immobilized nitrobacteria and aerobic denitrifying bacteria
LI Fang-fang1, QI Shu-ting2, SHI Yu-xin2, Lü Yu-shan1, GAO Chang-hong3
1. Biological Chemistry Department, Hebei University of Technology, Tianjin 300130, China; 2. Engineering Research Center of Seawater Utilization Technology, Ministry of Education, Hebei University of Technology, Tianjin 300130, China; 3. Graduate School of Hebei University of Technology, Tianjin 300130, China
Nitrobacteria and aerobic denitrifying bacteria were combined and immobilized in micro-organisms ball by adsorption-embedded -cross-linking method,using modified zeolite,polyvinyl alcohol,sodium alginate as immobilization carrier,and using boric acid and calcium chloride as crosslinking agent in this study.The removal rates of ammonia nitrogen,and accumulation level of nitrite and nitrate were measured under different combination ratios of bacteria.The removal rate of ammonia nitrogen was also determined under different conditions through the orthogonal experiment of four factors and three levels.Our results showed that ammonia nitrogen removal rate reached 82.38%when the ratio of nitrobacteria and aerobic denitrifying bacteria was 3:2,and that accumulation levels of nitrite and nitrate were 0.032 mg·L-1 and 0.053 mg·L-1,respectively.The highest removal rate of ammonia nitrogen reached 90.31%and occurred under the condition of zeolite dosage of 2 g·100 mL-1,temperature of 30℃,pH of 7.5,and shaking speed of 130 r·min-1. Furthermore,the micro-organisms ball made in our study had good mechanical properties and water absorption.
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