Sequence analysis of mitochondrial 12S rRNA gene in Oxyleotris
LI Chun-zhi1, ZHANG Bang-jie1, LI Ben-wang1, MO Jie-hua1, HUANG Yong-qiang1, ZHANG Rrui-yu2, ZHANG Qun3
1. Dongguan Fisheries Research Institute, Dongguan 523000; 2. Dongguan School of Economy &Trade, Gongguan 523000; 3. Institute of Hydrobiology, Jinan University, Guangzhou 510632, China
Oxyeleotris lineolatus、O.marmoratus and Odontobutis haifengensis are highly priced freshwater fishes,yet interspecific relationships haven't been reported yet.In present study,partial mitochondrial 12S rRNA gene of these 3 species were sequenced.Combined with 5 homologous 12S rRNA gene from the GeneBank,eight sequences were analyzed.The pairwise genetic distances were computed by Kimura 2-parameter model,and neighbor-joining based phylogenetic tree showed that Australia-originated O.lineolatus was a sister species to Tailand-endemic O.marmoratus.But Odontobutis haifengensis was distantly related to all other species,which is supporting the separation of Odontobutis from Eleotridae in traditional taxonomic treatment.Results showed that interspecific sequence divergence between O.lineolatus and O.marmoratus were obvious and no intraspecific 12s rRNA difference were observed,suggesting that mitochondrial 12S rRNA gene might be used as a useful molecular marker to identify different species in Eleotridae.
[2] Brown W M.1983.Evolution of animal mitochondrial DNAs[M].In Nei M,Koehned R K eds.Evolution of genes and proteins[M].Sinauer:Sunderland MA:Sinauer,62-88.
[3] Lu G,Li S.1998.Advances in the study and application of fish mitochondrial DNA polymorphism[J].Journal of Fisheries of China,3:94-103.
[4] Avise J.C.2000.Phylogeography:The History and Formation of Species[M].London:Harvard University Press,1-447.
[6] Hurng-Yi Wang,Mung-Pei Tsai,Jerry Dean,et.al.2001,Molecular phylogeny of Gobiood Fishes (Perciformes:Gobioidei) Based on Mitochondrial 12SrRNA Sequences[J].Molecular Phvlogenetics and Evolution,20:390-408.
[10] Honda M H,Ota M,Kobayashi,et.Al.1999.Phylogenetic relationship of Australian skinks of the Mabuya group (Reptilia:Scincidae) inferred from mitochondrial DNA sequence[J].Genes & Genetic Systems,74(4):135-139.
