眼点拟微绿球藻对抗生素的敏感性及其无菌藻株的培养

摘要
图/表
参考文献
相关文章 (4)

全文: PDF (384 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

研究了5种抗生素在不同浓度下对眼点拟微绿球藻(Nannochloropsis ocutala)生长的影响。结果表明:浓度为50μg·mL^-1时,氯霉素对眼点拟微绿球藻生长的抑制作用最明显,抑制率高达85.30%,2d和4d的Ic₅₀分别为293.64μg·mL^-1和67.69μg·mL^-1。其次为青霉素、硫酸庆大霉素和硫酸卡那霉素,4d的抑制率分别为8.95%、7.62%和3.84%,其中硫酸庆大霉素2d的Ic₅₀为316.40μg·mL^-1。链霉素对藻细胞生长的抑制作用最轻,抑制率仅为3%。按照抗生素对眼点拟微绿球藻生长抑制作用,由强到弱依次加入50μg·mL^-1青霉素,50μg·mL^-1硫酸庆大霉素,100μg·mL^-1硫酸卡那霉素和50μg·mL^-1链霉素,获得了无菌藻株。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	刘晓娟
	段舜山

关键词 ：眼点拟微绿球藻, 抗生素, 无菌藻株

Abstract：

Effects of 5 antibiotics(chloramphenicol,penicillin,streptomycin,kanamycin sulfate,gentamycini sulfate) at different concentrations on the growth of Nannochloropsis ocutala were studied.Results showed that at 50 μg·mL^-1,growth of N.ocutala was significantly inhibited by chloramphenicol,the highest inhibition was 85.30%,Ic₅₀ of 2 d and 4 d was 293.64 μg·mL^-1 and 67.69 μg·mL^-1,respectively.Followed by penicillin,gentamycini sulfate,and kanamycin sulfate,the inhibition rate of 4 d was 8.95%,7.62% and 3.84%,respectively.And 2 d Ic₅₀ of gentamycini sulfate was 316.40 μg·mL^-1.Growth of N.ocutala was not inhibited significantly by streptomycin,the inhibition rate was only 3%.According to susceptibility tests of antibiotics,a strategy of serial antibiotic treatments to obtain axenic culture was carried out:50 μg·mL^-1 penicillin,50 μg·mL^-1 gentamycini sulfate,100 μg·mL^-1 kanamycin sulphate,50 μg·mL^-1 streptomycin.

Key words： Nannochloropsis ocutala antibiotics axenic culture

收稿日期: 2006-12-25

:	Q938
	8

基金资助:国家自然科学基金资助(30370231);珠海市科技计划重大项目(PB20041018);广东省科技计划重点引导项目(2005B33201001)

引用本文:

刘晓娟, 段舜山*. 眼点拟微绿球藻对抗生素的敏感性及其无菌藻株的培养[J]. , 2006, 25(6): 493-495.
LIU Xiao-juan, DUAN Shun-shan. Study on the susceptibility tests of antibiotics and obtain axenic culture of Nannochloropsis ocutala. , 2006, 25(6): 493-495.

链接本文:

http://www.ecolsci.com/CN/Y2006/V25/I6/493

[1] Chen F.1996.High cell density culture of microalgae in heterotrophic growth[J].Trends in Biotechnology,14:421-426.

[2] Markager S,Jensen K S.1990.Heterotrophic growth of Ulvalactuca(Chlorophyceae)[J].Journal of Phycology,26:670-673.

[3] Kitano M,Matsukawa R,Karube I.1997.Changes in eicosapentaenoic acid content of Navicula saprophila,Rhodomonas salina and Nitzschia sp.under mixotrophic conditions[J].Journal of Applied Phycology.9:559-563.

[4] Xie J L,Zhang Y X,Li Y G.2001.Mixotrophic cultivation of Platymonas subcordiformis[J].Journal of Applied Phycology,13:343-347.

[5] 潘欣,李建宏,戴传超,等.2002.小球藻异养培养的研究[J].食品科学,4(23):28-33.

[6] Imai I,Yamaguchi M.1994.A simple technique for establishing axenic cultures of phytoflagellates[J].Bull Jpn Soc Microb Ecol,9:15-17.

[7] 张士璀,马军英,范晓.1997.海洋生物技术原理与应用[M].海洋出版社.90-91.

[8] Droop M R.1967.A procedure for routine purification of algal cultures with antibiotics[J].Br Phycol Bull.3:295.297.

[9] Divan C L,Schnoes H K.1982.Production of Axenic Gonyaulax Cultures by Treatment with Antibiotics[J].Applied and environmental microbiology,1(44):250-254.

[10] Cottrell M T,Suttle C A.1993.Production of axenic cultures of Micromonas pusilla(Pracinophyceae) using antibiotics[J].Journal of Phycology,29:385-387.

[11] Yim J H,Lee H K.2004.Axenic Culture of Gyrodinium impudicum Strain KG03.a Marine Red-tide Microalga that Produces Exopolysaccharide[J].The Journal of Microbiology,4(42):305-314.

[12] 陈绍铭,郑福寿.1985.水生微生物实验法[M].海洋出版社.35,139.

[13] 尚迅,戴继勋,韩宝芹,等.2000.颤藻对6种抗生素敏感性的研究[J].海洋学报,22(6):131-135.

[14] 郑合明,薛乐勋.2002.抗生素及草酊磷对盐藻生长的影响[J].郑州大学学报,37(6):790-793.

[15] Martínez G V,Rodriguezc M H,Hernándezd F H,et al.2004.Strategy to obtain axenic cultures from field-collected samples of the cyanobacterium Phormidium animalis[J].Journal of Microbiological Methods.57:115-121.

[16] 林伟.2000.几种海洋微藻的无菌化培养[J].海洋科学,24(10):4-6.