Detection of DNA Damages in Hemocytesof A. woodiana woodiana Induced by Cadmium with single Cell Gel electrophoresis Assay
ZHOU Kan, OUYANG Shan, WU Xiao-ping, WU Tian
College of Life Sciences, Nanchang University, Nanchang; The Key Lab. of Poyang Lake Ecology and Bioresource Utilization, Ministry of Education.China, Nanchang University, Nanchang 330031, China
In this study,mussels(Anodonta woodiana woodiana) were chosen as the research object.We assessed the possible DNA damage in haemocytes of mussels using the single cell gel electrophoresisassay.Results showed haemocytes images which exposed to cadimium presented comet tails,compared the exposed groups with negative control group,existed significant differences.Mussels were exposed to different cadmium chloride concentrations(0,1,10,50 mg·L-1) respectively for 72 hours,the results showed that the average length of DNA migration and tai lDNA% in all exposed groups was significantly higher than that of negative control group(p<0.01) and existed significant dose-effect relationship.Otherwise,at the concentration of 10 mg·L-1,four time-effect groups(24,48,72,96 h) and a zero time control group were set up,the average length of DNA migration and tail DNA% under different exposed times in cadmium chloride was significantly higher than zero time group(p<0.01),but no significant time-effect relationship among the tested concentrations.
周侃, 欧阳珊, 吴小平*, 吴甜. SCGE技术检测镉对背角无齿蚌血细胞DNA的损伤[J]. , 2008, 27(2): 90-94.
ZHOU Kan, OUYANG Shan, WU Xiao-ping, WU Tian. Detection of DNA Damages in Hemocytesof A. woodiana woodiana Induced by Cadmium with single Cell Gel electrophoresis Assay. , 2008, 27(2): 90-94.
[1] 刘洪伟,印明昊,张峰.2005.重金属污染对水生生物免疫毒性的研究[J].中国水产,2:76-78.
[2] 周惜时,廖柏寒,秦普丰,等.2006.表面活性剂与重金属对泥鳅的联合毒性研究[J].水利渔业,26(2):86-88.
[3] 丁磊,黄鹤忠,吴康,等.2005.镉致鲫(Carassius auratus)外周血单个核细胞DNA损伤与增殖抑制[J].海洋与湖沼,36(1):62-65.
[4] 胡晓磐,周建华,时夕金.2005.利用单细胞凝胶电泳技术研究镉对鲫鱼淋巴细胞DNA的损伤[J].农业环境科学学报,24(1):43-45.
[5] 朱国念,周新文,孙锦荷.2003.混合重金属离子诱导的鲫鱼DNA损伤与基因表达[J].核农学报,17(3):199-202.
[6] Emmanouil C,Sheehan T M T,Chipmana J k 2007.Macromolecule oxidation and DNA repair in mussel(Mytilus edulis L.)gill following exposure to Cd and Cr(VI).Aquatic Toxicology,82:27-35.
[7] Juhe G,O'Halloran J,Culloty S C.2007.In Vivo Exposure to Microcystins Induces DNA Damage in the Haemocytes of the Zebra Mussel,Dreissena polymorpha,as Measured With the Comet Assay[J].Environmental and Molecular Mutagenesis.48:22-29.
[8] Pavlica M,Klobuear G I V,Mojas N.2001.Detection of DNA damage in haemoeytes of zebra mussel using comet assay[J].Genetic Toxicology and Environment Mutagenesis,490:209-214.
[9] Michel L G Amy H R,Walter W P.2003.Detection of oxidative DNA damage in isolated marine bivalve hemocytes using the comet assay and formamido pyrimidine glycosylase[J].Genetic Toxicology and Environment Mutagenesis,54(2):15-22.
[10] Gabbianelli R Moretli M,Carpcne E.2006.Effect of different organotins on DNA of mollusk(Scapharca inaequivalvis)erythroytes assessed by the comet assay[J].Science of the Total Environment,367:163-169.
[11] Villela I V,Oliveira I M,Silva J d.2006.DNA damage and repair in haemolymph cells of golden mussel(Limnoperna fortunei)exposed to environmental contaminants[J].Mutton Research,605:78-86.
[12] Singh N P,Mccoy M L,Tice R R,et al.1988.A simple technique for quantitation of low levels of DNA damage in individual cell[J].Exp Cell Res,175(2):184-191.
[13] Lee R.F Steinen S.2003.Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic(marine and freshwater)animals[J].Mutation Research,544:43-64.
[14] Zhou B S,Liu W H,Siu William H L.2006.Exposure of spermatozoa to duroquinone may impair reproduction of the common carp(Cyprinus carpio)through oxidative stress[J].Aquatic Toxicology,77:136-142.
[15] 蔡颖,庄志雄.1999.镍和镉对人外周血淋巴细胞的DNA损伤作用[J].中华预防医学杂志,33(2):75-77.
[16] Lankoff A,Wojcik A,Fcssard V.2006.Nodularin-induced genotoxicity following oxidative DNA damage and aneuploidy in HepG2 cells[J].Toxicology Letters,164:239-248.
[17] 厉以强,孙立伟,曲甍甍,等.2004.氨基酚类化合物对鲤鱼(Cyprinus caprio)肾细胞遗传毒性的影响[J].环境科学研究,5(17):35-37.
[18] Vanzella T P,Martincz C B R,C'olus I M S.2007.Genotoxic and mutagenic effects of diesel oil water soluble fraction on a neotropical fish species[J].Mutation Research,631:36-43.
