1. 华南师范大学生命科学学院, 广州, 510631; 2. 暨南大学生命科学技术学院, 广州, 510632; 3. Department of Plant Physiology, Lund University, Box 117, SE-22100 Lund, Sweden
Temperature effects on the formation of DNA damage in Nicotiana tabacum leaf discs induced by UV-B Irriadiation
LiShaoshan, Wang Yan, Lars Olof Bjorn
1. School of Life Science, South China Normal University, Guangzhou 510631, China; 2. School of Life Science and 4 Technology, Jinan University, Guangzhou 510632, China; 3. Department of Plant Physiology, Lund University, Box 117, SE-22100 Lund, Sweden
Cyclobutane pyrimidine dimers(CPD) and 6-4 photoproducts(6-4PP) are two major kinds of photolesion induced by UV-B radiation in plants. By using ELISA method of with specific monoclonal antibodies, UV-B induced formation of CPD and 6-4PP in tobacco leaf discs at different temperatures was investigated. CPD increased during 4 h UV-B exposure. UV-B radiation induced formation of CPD and 6-4PP even at 0℃, but low temperature resulted in less damage. Contrary to the commonly accepted view that DNA damage induced by UV-B is temperature-independent because of its photochemical nature, we have thus found that UV-B-induced formation of CPD and 6-4PPin tobacco leaf discs is temperature-dependent.
李韶山1, 王艳2*, Lars Olof Bjorn3. 温度对UV-B诱导的烟草叶圆片DNA损伤的影响[J]. , 2002, 21(2): 115-117.
LiShaoshan, Wang Yan, Lars Olof Bjorn. Temperature effects on the formation of DNA damage in Nicotiana tabacum leaf discs induced by UV-B Irriadiation. , 2002, 21(2): 115-117.
[1] Bjom L O, Callaghan T V, Gehrke C, Johanson U, Sonesson M, 1999. Ozone depletion, ultraviolet radiation and plant life. Chemo sphere:Global Changes Science 1:449-454, Pergamon
[2] Britt A 1999. Molecular genetics of DNA repair in high plants[J]. Trends Plant Sci, 4(1):20-25
[3] Li SS(李韶山),Wang Y(王艳),Wang XJ(王小菁),Bin JH(宾金华),Liu SH(刘颂豪)2000.Cyclobutane pyrimidine dimeraccumulation in relation to UV-B sensitivity in rice cultivars. ActaBot Sin(植物学报),42:576-581
[4] Li SS(李韶山),Paulsson M, Bjom L O 2002. Temperature-dependent formation and photorepair of DNA damage induced by UV-B radiation in suspension-cultured tobacco cells[J]. J Pho tochem Photobiol B:Biol, 66(1):67-72
[5] Mori T, Nakane M, Hattori T, Matsunaga T, Ihara M, Nikaido O 1991. Simultaneous establishment of monoclonal antibodies specific for either cyclobutane pyrimidine dimer or(6-4) photoproduct from the same mouse immunized with ultraviolet-irradiated DNA[J] Photochem Photobiol, 54:225-232
[6] Pakker H, Martins R, Boelen P, Buma A, Nikaido O, Breeman A M. 2000. Effects of temperature on the photoreactivation of ultravio et-B-induced DNA damage in Palmaria palmata(Rhodophyta)[J].J. Phycol, 36:334-341
[7] Stapleton A, Thornber C, Walbot V. 1997. UV-B component of sunlight causes measurable damage in field-grown maize(Zea mays L.):developmental and cellular heterogeneity of damage and repair[J]. Plant Cell and Environ, 20:279-290
[8] Takeuchi Y, Murakami M, Nakajima N, Kondo N, Nikaido O 1996. Induction and repair of damage to DNA in cucumber cotyledons irradi ated with UV-B[J]. Plant Cell Physiol, 37:181-187
