To explore the effects of cytokinin on the growth and negative phototropism of Chlorophytum comosum var.variegata root, some healthy plants of Chlorophytum comosum var.variegata were selected and cultivated in different concentration of 6-BA solution with unilateral light illuminated,and the growth rate and the negative inclination of the root of chlorophytum comosum were measured. The results showed that:(1) 6-BA inhibited the growth of both the adventitious root and the branch root growing out from the adventitious root,decreased the bend angle of the negative phototropism of all of the elongated root,the inhibited degree was intensified with the increase of 6-BA concentration.(2)The adventitious root ceased to elongation when treated for 7 days with 10mg·L-1 6-BA or for 5 days with 100mg·L-1 6-BA;(3) All the branch root growing out from the adventitious root could not extend but form much little bump from the surface of the adventitious root when treated with 10-100 mg·L-1 6-BA,the amount of the bump from the surface of the adventitious root were increased with the rise the concentration of 6-BA;(4) The negative phototropism of control was remarkable and inclination angle was near 90°,but inclination angle decreased to about 15°when treated with100mg·L-1 6-BA.The possible mechanism was discussed in this study.
[1] 江玲,管小春.植物激素与不定根的形成[J].生物学通报,2000,35(11):17-19
[2] De Smet I,Tetsumura T,De Rybel B,Frey N F,Laplaze L,Casimiro I,Swamp N M,Vanneste S,Audenaert D,Inze D,Bennett M J,Beeckman T.Auxin-dependent regulation of lateral root positioning in the basal meristem of Arabidopsis[J].Development(Cambridge,England).2007,134:681-690.
[3] Riefler M,Novak O,Stmad M,Schmiilling T.Arabidopsis cytokinin receptor mutants reveal functions in shoot growth,leaf senescence,seed size,germination,root development and cytokinin metabolism[J].Plant Cell,2006,18:40-54.
[4] Wang Z,Mo Y W,Qian S Q,Gu Y J.Negative phototropism of rice root and its influencing factors[J].Science in China(Ser C),2002,45:485-496.
[5] 韩鹰,高玥,王忠.水培球根花卉根的负向光性与根系造型[J].植物生理学通讯,2006,42(3):511-514.
[6] 范玉琴,吴辉,黄思梅,刘惠娜.向日葵种子根负向光反应部位的初步研究[J].内江科技,2007,26(4):233-235.
[7] 陈娟,王忠,孔妤,顾蕴洁,莫亿伟,蔡唱,孙鉴坤.植物根负向光性反应[J].植物生理学通讯,2009,45:299-304.
[8] 莫亿伟,王忠,钱善勤,顾蕴洁.生长素在水稻负向光性反应中的作用[J].中国水稻科学,2004,18(3):245-248.
[9] 段留生,张一,何钟佩,翟志席.生长素和细胞分裂素对棉花幼苗根系顶端优势的调控作用[J].棉花学报,2001,13(6):330-333.
[10] Werner T,Motyka V,Laucou V.Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity[J].Plant Cell,2003,15:2532-2550.
[11] Bollmark M,Elmsson L.Effects of exogenous cytokinins on root formation in pea cuttings[J].Physiologia Plantarum,1986,68:662-666.
[12] Skoog F,Miller CO.Chemical regulation of growth and organ formation in plant tissue cultured in vitro.Symposium Society of Experimental Biology,1957,11:118-131
[13] Kaufman LS.Transduction of blue-light signals[J].Plant Physiology,1993,102(2):333-337
[14] 余让才,潘瑞炽.蓝光对水稻幼苗生长及内源激素水平的影响[J].植物生理学报,1997,23:175-180
[15] Laplaze L,Benkova E,Casimiro I,MaesL,Vanneste S,Swarup R,Weijers D,Calvo,V,Parizot B,Herrera-Rodriguez MB.Cytokinins actdirectly on lateral root founder cells to jnhibit root initiation[J].The Plant Cell,2007.19:3889-3900.
