三种环境激素对四种海洋微藻的急性毒性效应研究

摘要
图/表
参考文献
相关文章 (13)

全文: PDF (444 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

近年来,海洋环境激素污染日益严重,为了考察环境激素对海洋微藻的生物毒性效应,进而评估其对海洋生态系统的影响,该实验研究了三氯卡班、邻苯二甲酸二丁酯、三丁基氯化锡三种环境激素对海洋小球藻(Chlorella sp.)、眼点拟微绿球藻(Nannochloropsis ocutala)、球形棕囊藻(Phaeocystis globosa)和东海原甲藻(Prorocentrum donghaiense )4种海洋微藻的急性毒性效应。结果表明,三种环境激素均可显著抑制该4种微藻的生长。三氯卡班对4种微藻的96 h-EC₅₀分别为108.19 μg·L^-1、63.21 μg·L^-1、60.73 μg·L^-1和57.58 μg·L^-1;邻苯二甲酸二丁酯对4种微藻的96 h-EC₅₀分别为1.42 mg·L^-1、1.02 mg·L^-1、1.47 mg·L^-1 和1.21 mg·L^-1;三丁基氯化锡对4种微藻的96 h-EC₅₀分别为3.5 μg·L^-1、4.36 μg·L^-1、0.6 μg·L^-1和0.6 μg·L^-1。三种环境激素对四种海洋微藻的毒性强弱顺序为三丁基氯化锡>三氯卡班>邻苯二甲酸二丁酯。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	田斐
	何宁
	段舜山

关键词 ：三氯卡班, 邻苯二甲酸二丁酯, 三丁基氯化锡, 海洋微藻, 急性毒性

Abstract：

Environmental hormone pollution is becoming more and more serious in marine environment recently. In order to evaluate the impact of environmental hormones on marine eco-system, the acute toxic effect of three environmental hormones on four marine microalgae was investigated. Results showed that the growth of microalgae including Chlorella sp., Nannochloropsis ocutala, Phaeocystis globosa and Prorocentrum donghaiense was significantly inhibited by the three environmental hormones. The 96 h median effective concentrations (96 h-EC₅₀) of triclocarban for Chlorella sp., N. ocutala, P. globosa and P. donghaiense were 108.19 μg·L^-1, 63.21 μg·L^-1, 60.73 μg·L^-1 and 57.58 μg·L^-1, respectively. The values of 96 h-EC₅₀ of dibutyl phthalate for four microalgae were 1.42 mg·L^-1, 1.02 mg·L^-1, 1.47 mg·L^-1 and 1.21 mg·L^-1, and those of tributyl tin chloride for four microalgae were 3.5 μg·L^-1, 4.36 μg·L^-1, 0.6 μg·L^-1 and 0.6 μg·L^-1, respectively. The toxic intensity of the three environmental hormones to marine microalgae was in the order of tributyl tin chloride > triclocarban > dibutyl phthalate.

Key words： triclocarban dibutyl phthalate tributyl tin chloride marine microalgae acute toxicity

收稿日期: 2013-08-25

X171.5

基金资助:NSFC-广东联合基金重点项目(U1133003)和国家自然科学基金项目(41176104)

引用本文:

田斐, 何宁, 段舜山*. 三种环境激素对四种海洋微藻的急性毒性效应研究[J]. , 2013, 32(4): 401-407.
TIAN Fei, HE Ning, DUAN Shun-shan*. Acute toxic effects of environmental hormones on marine microalgae. , 2013, 32(4): 401-407.

链接本文:

http://www.ecolsci.com/CN/Y2013/V32/I4/401

[1] Cai Q Y, Mo C H, Wu Q T, Zeng Q Y, Katsoyiannis A. Occurrence of organic contaminants in sewage sludges from eleven wastewater treatment plants, China[J]. Chemosphere, 2007, 68(9): 1751-1762.
[2] Xu J, Wang P, Guo W F, Dong J X, Wang L, Dai S G. Seasonal and spatial distribution of nonylphenol in Lanzhou Reach of Yellow River in China[J]. Chemosphere, 2006, 65(9): 1445-1451.
[3] Yang L H, Luan T G, Lan C Y. Solid-phase microextraction with on-fiber silylation for simultaneous determinations of endocrine disrupting chemicals and steroid hormones by gas chromatography-mass spectrometry[J]. Journal of Chromatography A, 2006, 1104(1-2): 23-32.
[4] Deng J, Liu C S, Yu L Q, Zhou B S. Chronic exposure to environmental levels of tribromophenol impairs zebrafish reproduction[J]. Toxicology and Applied Pharmacology, 2010, 243(1): 87-95.
[5] Liu W, Zhang Y B, Quan X, Jin Y H, Chen S. Effect of perfluorooctane sulfonate on toxicity and cell uptake of other compounds with different hydrophobicity in green alga[J]. Chemosphere, 2009, 75(3): 405-409.
[6] Zhang B Z, Ni H G, Guan Y F, Zeng E Y. Occurrence, bioaccumulation and potential sources of polybrominated diphenyl ethers in typical freshwater cultured fish ponds of South China[J]. Environmental Pollution, 2010, 158(5): 1876-1882.
[7] WHO. Guidelines for Drinking-water Quality Di (2-ethylhexyl) phthalate in Drinking-water[R]. WHO/SDE/WSH/03.04/29. Geneva: World Health Organization, 1996.
[8] Jia X W, Zhang Z P, Wang G D, Zou Z H, Wang S H, Huang B Q, Wang Y L. Expressed sequence tag analysis for identification and characterization of genes related to Tributylin (TBT) exposure in the abalone Haliotis diversicolor supertexta[J]. Comparative Biochemistry and Physiology, Part D, 2009, 4(4): 255-262.
[9] Wade T L, Sweet S T, Quinn J G, Cairns R W, King J W. Tributyltin in environmental samples from the Former Derecktor Shipyard, Coddington Cove, Newport RI[J]. Environmental Pollution, 2004, 129(2): 315-320.
[10] Békri K, Saint-Louis R, Pelletier É. Determination of tributyltin and 4-hydroxybutyldibutyltin chlorides in seawater by liquid chromatography with atmospheric pressure chemical ionization-mass spectrometry[J]. Analytica Chimica Acta, 2006, 578(2): 203-212.
[11] Rodríguez J G, Solaun O, Larreta J, Segarra M J B, Franco J, Alonso J I G, Sariego C, Valencia V, Borja Á. Baseline of butyltin pollution in coastal sediments within the Basque Country (northern Spain), in 2007-2008[J]. Marine Pollution Bulletin, 2010, 60(1): 139-145.
[12] Zhou X L, Ye X Y, Calafat A M. Automated on-line column-switching HPLC-MS/MS method for the quantification of triclocarban and its oxidative metabolites in human urine and serum[J]. Journal of Chromatography B, 2012, 881-882: 27-33.
[13] Snyder E H, O'Connor G A, McAvoy D C. Toxicity and bioaccumulation of biosolids-borne triclocarban (TCC) in terrestrial organisms[J]. Chemosphere, 2011, 82(3): 460-467.
[14] Silva A, Figueiredo S A, Sales M G, Delerue-Matos C. Ecotoxicity tests using the green algae Chlorella vulgaris-A useful tool in hazardous effluents management[J]. Journal of Hazardous Materials, 2009, 167(1-3):179-185.
[15] Tamura I, Kagota K, Yasuda Y, Yoneda S, Morita J, Nakada N, Kameda Y, Kimura K, Tatarazako N, Yamamoto H. Ecotoxicity and screening level ecotoxicological risk assessment of five antimicrobial agents: triclosan, triclocarban, resorcinol, phenoxyethanol and p-thymol[J]. Journal of Applied Toxicology, 2012.
[16] Sidharthan M, Young K S, Woul L H, Soon P K, Shin H W. TBT toxicity on the marine microalga Nannochloropsis oculata[J]. Marine Pollution Bulletin, 2002, 45(1-12): 177-180.
[17] Fernández-Alba A R, Hernando M D, Piedra L, Chisti Y. Toxicity evaluation of single and mixed antifouling biocides measured with acute toxicity bioassays[J]. Analytica Chimica Acta, 2002, 456(2): 303-312.
[18] Perron M C, Juneau P. Effect of endocrine disrupters on photosystem II energy fluxes of green algae and cyanobacteria[J]. Environmental Research, 2011, 111(4): 520-529.
[19] Chen Zhen, Juneau P, Qiu B S. Effects of three pesticides on the growth, photosynthesis and photoinhibition of the edible cyanobacterium Ge-Xian-Mi (Nostoc)[J]. Aquatic Toxicology, 2007, 81(3): 256-265.
[20] Qian H F, Pan X J, Shi S T, Yu S Q, Jiang H Y, Lin Z F, Fu Z W. Effect of nonylphenol on response of physiology and photosynthesis-related gene transcription of Chlorella vulgaris[J]. Environmental Monitoring and Assessment, 2011, 182(1-4): 61-69.
[21] Elbaz A, Wei Y Y, Meng Q, Zheng Q, Yang Z M. Mercury-induced oxidative stress and impact on antioxidant enzymes in Chlamydomonas reinhardtii[J]. Ecotoxicology, 2010, 19(7): 1285-1293.
[22] Cloern J E, Jassby A D, Thompson J K, Hieb K A. A cold phase of the East Pacific triggers new phytoplankton blooms in San Francisco Bay[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(47): 18561-18565.
[23] 国家环境保护总局. 新化学物质危害评估导则(HJ/T154-2004)[M]. 北京: 中国环境科学出版社, 2004.
[24] Halden R U, Paull D H. Co-occurrence of triclocarban and triclosan in U.S. water resources[J]. Environmental Science & Technology, 2005, 39(6): 1420-1426.
[25] Jiang G B, Zhou Q F, Liu J Y, Wu D J. Occurrence of butyltin compounds in the waters of selected lakes, rivers and coastal environments from China[J]. Environmental Pollution, 2001, 115(1): 81-87.